Supplementary information
From the following article:
SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis
Stephen P. Grigg, Claudia Canales, Angela Hay & Miltos Tsiantis
Nature 437, 1022-1026(13 October 2005)
doi:10.1038/nature04052
Supplemntary Figure S1
A schematic of the SE genomic locus indicating the positions of lesions in se mutant alleles, and contains information regarding transcripts produced by these alleles.
Supplementary Figure S2
RT-PCR and RNA gel blot analysis of expression of various transcripts in se-3 and Col, and contains descriptions of PCR conditions and primer sequences.
Supplementary Figure S3
A histogram reflecting the cytosine methylation status of a region of the PHB genomic locus in se-3 and Col. Clones generated from se-3 show reduced levels of CpG methylation.
Supplementary Figure S4
Gene expression analysis of the gibberellic acid biosynthetic gene GA5, demonstrating that this gene is similarly regulated in a KNOX gain-of-function line, a dominant phb mutant, and se-1.
Supplementary Methods
Experimental procedures used in generation of the supporting online material not included in the main methods section.
Supplementary Table S1
A sequence alignment of all clones analysed for generating bisulphite sequencing data presented in Supplementary Figure S3.
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