Supplementary information

From the following article:

Atomic model of a myosin filament in the relaxed state

John L. Woodhead, Fa-Qing Zhao, Roger Craig, Edward H. Egelman, Lorenzo Alamo & Raúl Padrón

Nature 436, 1195-1199(25 August 2005)

doi:10.1038/nature03920

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Supplementary Figure S1

Longitudinal view of reconstruction density seen in projection, demonstrating arrowheads (cf. Fig. 1a of paper)

Supplementary Figure S2

Longitudinal surface view of reconstruction, showing how the transverse view in Fig. 2a of paper is obtained (see Supplementary Movies S2 and S3).

Supplementary Figure S3

Transverse view of reconstruction seen in projection, demonstrating high density of subfilaments and low density of filament core (cf. Fig. 2b of paper).

Supplementary Movie S1

Movie of longitudinal view of reconstruction rotated around long axis of filament (cf. Fig. 1c of paper).

Supplementary Movie S2

Movie showing how longitudinal view of reconstruction transforms into transverse view seen in Fig. 2a of paper.

Supplementary Movie S3

This movie shows how portions of different crowns contribute to the transverse view of the reconstruction containing a single 14.5 nm repeat seen in Fig. 2a of paper.

Supplementary Movie S4

This movie provides a three-dimensional perspective on the fitting of the modified smooth muscle HMM atomic structure into the J-motif of the filament reconstruction (cf. Fig. 3a of paper).

Supplementary Movie S5

This movie shows different views of the space filling atomic model of PDB 1i84 after modification to fit the "J" motif of the reconstruction (cf. Figs 3b and 5a of paper).

Supplementary Movie S6

Movie showing how S2 fits into the reconstruction (cf. Fig. 4 of paper).

Supplementary Movie Legends

Text to accompany the above Supplementary Movies.

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