Abstract
THE leucine-rich repeat is a recently characterized structural motif1 used in molecular recognition processes as diverse as signal transduction, cell adhesion, cell development, DNA repair and RNA processing2. We present here the crystal structure at 2.5 Å resolution of the complex between ribonuclease A and ribonculease inhibitor, a protein built entirely of leucine-rich repeats. The unusual non-globular structure of ribonuclease inhibitor, its solvent-exposed parallel β-sheet and the conformational flexibility of the structure are used in the interaction; they appear to be the principal reasons for the effectiveness of leucine-rich repeats as protein-binding motifs. The structure can serve as a model for the interactions of other proteins containing leucine-rich repeats with their ligands.
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References
Takahashi, N., Takahashi, Y. & Putnam, F. W. Proc. natn. Acad. Sci. U.S.A. 82, 1906–1910 (1985).
Kobe, B. & Deisenhofer, J. Trends biochem. Sci. 19, 415–421 (1994).
Hofsteenge, J., Kieffer, B., Matthies, R., Hemmings, B. A. & Stone, S. R. Biochemistry 27, 8537–8544 (1988).
Kobe, B. & Deisenhofer, J. Nature 366, 751–756 (1993).
Avey, H. P. et al. Nature 11, 557–562 (1967).
Kartha, G., Bello, J. & Harker, D. Nature 213, 862–865 (1967).
Borkakokti, N. Eur. J. Biochem. 132, 89–94 (1983).
Birdsall, D. L. & McPherson, A. J. biol. Chem. 267, 22230–22236 (1992).
Nachman, J. et al. Biochemistry 29, 928–937 (1990).
CCP4 Acta crystallogr. D50, 760–763 (1994).
Janin, J. & Chothia, C. J. biol. Chem. 265, 16027–16030 (1990).
Vicentini, A. M. et al. Biochemistry 29, 8827–8834 (1990).
Roth, J. S. Biochim. biophys. Acta 21, 34–43 (1956).
Fominaya, J. M. & Hofsteenge, J. J. biol. Chem. 267, 24655–24660 (1992).
Wlodawer, A., Svensson, L. A., Sjolin, L. & Gilliland, G. L. Biochemistry 27, 2705–2717 (1988).
Yoder, M. D., Keen, N. T. & Jurnak, F. Science 260, 1503–1507 (1993).
Baumann, U., Wu, S., Flaherty, K. M. & McKay, D. B. EMBO J. 12, 3357–3364 (1993).
Steinbacher, S. et al. Science 265, 383–386 (1994).
Davies, D. R. & Padlan, E. A. Curr. Biol. 2, 254–256 (1992).
Kobe, B & Deisenhofer, J. J. molec. Biol. 231, 137–140 (1993).
Kobe, B., Ma, Z. & Diesenhofer, J. J. molec. Biol. 241, 288–291 (1994).
Otwinowski, Z. Proc. CCP4 Study Weekend (eds Sawyer, L. et al.) 56–62 (SERC, Daresbury Laboratory, Daresbury, UK 1993).
Brünger, A. T., Kuriyan, J. & Karplus, M. Science 235, 458–460 (1987).
Jones, T. A., Bergdoll, M. & Kjeldgaard, M. Crystallography and Modeling Methods in Molecular Design (eds Bugg, C. E. & Ealick, S. E.) 189–195 (Springer, New York, 1990).
Brünger, A. T. Nature 355, 472–475 (1992).
Luzzati, P. V. Acta crystallogr. 5, 802–810 (1952).
Read, R. J. Acta crystallogr. A42, 140–149 (1986).
Carson, M. J. molec. Graphics 5, 103–106 (1987).
Nicholls, A. & Honig, B. J. comp. Chem. 12, 435–445 (1991).
Kraulis, P. J. appl. Crystallogr. 24, 946–950 (1991).
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Kobe, B., Deisenhofer, J. A structural basis of the interactions between leucine-rich repeats and protein ligands. Nature 374, 183–186 (1995). https://doi.org/10.1038/374183a0
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DOI: https://doi.org/10.1038/374183a0
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