Abstract
RNAs that contain specific high-affinity binding sites for small molecule ligands immobilized on a solid support are present at a frequency of roughly one in 1010-1011 in pools of random sequence RNA molecules1,2. Here we describe a new in vitro selection procedure designed to ensure the isolation of RNAs that bind the ligand of interest in solution as well as on a solid support. We have used this method to isolate a remarkably small RNA motif that binds ATP, a substrate in numerous biological reactions and the universal biological high-energy intermediate. The selected ATP-binding RNAs contain a consensus sequence, embedded in a common secondary structure. The binding properties of ATP analogues and modified RNAs show that the binding interaction is characterized by a large number of close contacts between the ATP and RNA, and by a change in the conformation of the RNA.
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References
Ellington, A. D. & Szostak, J. W. Nature 346, 818–822 (1990).
Famulok, M. & Szostak, J. W. J. Am. chem. Soc. 114, 3990–3991 (1992).
Arnold, F. H., Scofield, S. A. & Blanch, H. W. J. Chromatogr. 355, 1–12 (1986).
Tuerk, C. et al. Proc. natn. Acad. Sci. U.S.A. 85, 1364–1368 (1988).
Fersht, A. in Enzyme Structure and Mechanism 186–188 (Freeman, New York, 1985).
Moazed, D. & Noller, H. F. J. molec. Biol. 187, 399–416 (1986).
Rebek, J. Jr Science 235, 1478–1484 (1987).
Echavarren, A., Galan, A., Lehn, J-M. & de Mendoza, J. J. Am. chem. Soc. 111, 4994–4995 (1989).
Breslow, R., Greenspoon, N., Guo, T. & Zarzycki, R. J. chem. Soc. 111, 8296–8297 (1989).
Goswami, S. & Hamilton, A. D. J. Am. chem. Soc. 111, 3425–3426 (1989).
Chang, S-K., Van Engen, D., Fan, E. & Hamilton, A. D. J. Am. chem. Soc. 113, 7640–7645 (1991).
Carcanague, D. R., Knobler, C. B. & Diederich, F. J. Am. chem. Soc. 114, 1515–1517 (1992).
Fan, E., Van Arman, S. A., Kincaid, S. & Hamilton, A. W. J. Am. chem. Soc. 115, 369–370 (1993).
Yoon, S. S. & Still, W. C. J. Am. chem. Soc. 115, 823–824 (1993).
Rotello, V. M., Viani, E. A., Deslongchamps, G., Murray, B. A. & Rebek, J. Jr J. Am. chem. Soc. 115, 797–798 (1993).
Joyce, G. Nature, 338, 217–224 (1989).
Benner, S. A., Ellington, A. D. & Tauer, A. Proc. natn. Acad. Sci. U.S.A. 86, 7054–7058 (1989).
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Sassanfar, M., Szostak, J. An RNA motif that binds ATP. Nature 364, 550–553 (1993). https://doi.org/10.1038/364550a0
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DOI: https://doi.org/10.1038/364550a0
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