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Release of secretory products during transient vesicle fusion

Nature volume 363, pages 554–558 (1993)Cite this article

Abstract

PATCH-CLAMP experiments have shown that fusion of secretory granules with the plasma membrane does not always occur as an all-or-none event, but can develop slowly in a fluctuating manner or can be transient1–5. These observations suggested that release could be detected during such incomplete fusion events. To test this hypothesis we have combined patch-clamp measurements of the activity of single exocytotic fusion pores in beige mouse mast cells with the electrochemical detection of serotonin released during the exocytotic events. We report here that on fusion pore opening there is a small release of serotonin which is directly proportional to the pore conductance. We also show that a significant release occurs during transient fusion events. These results demonstrate, to our knowledge for the first time, release of a neurotransmitter from a secretory vesicle that did not undergo complete fusion.

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References

  1. Fernández, J. M., Neher, E. & Gomperts, B. D. Nature 312, 453–455 (1984).

    Article  ADS  Google Scholar 

  2. Breckenridge, L. & Almers, W. Nature 328, 814–817 (1987).

    Article  ADS  CAS  Google Scholar 

  3. Zimmerberg, J., Curran, M., Cohen, F. S. & Brodwick, M. Proc. natn. Acad. Sci. U.S.A. 84, 1585–1589 (1987).

    Article  ADS  CAS  Google Scholar 

  4. Breckenridge, L. & Almers, W. Proc. natn. Acad. Sci. U.S.A. 84, 1945–1949 (1987).

    Article  ADS  CAS  Google Scholar 

  5. Alvarez de Toledo, G. & Fernández, J. M. Cell Physiology of Blood (eds Gunn, R. B. & Parker, J. C.) 333–344 (The Rockefeller University Press, New York, 1988).

    Google Scholar 

  6. Hamill, O. P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F. J. Pflügers Arch. 391, 85–100 (1981).

    Article  CAS  Google Scholar 

  7. Neher, E. & Marty, A. Proc. natn. Acad. Sci. U.S.A. 79, 6712–6716 (1982).

    Article  ADS  CAS  Google Scholar 

  8. Wightman, R. M. et al. Proc. natn. Acad. Sci. U.S.A. 88, 10754–10758 (1991).

    Article  ADS  CAS  Google Scholar 

  9. Tatham, P. E. R., Duchen, M. & Millar, J. Pflügers Arch. 419, 409–414 (1991).

    Article  CAS  Google Scholar 

  10. Chow, R. H., von Rüden, L. & Neher, E. Nature 356, 60–63 (1992).

    Article  ADS  CAS  Google Scholar 

  11. Fernández-Chacón, R. & Alvarez de Toledo, G. Biophys. J. 61, A226 (1992).

    Google Scholar 

  12. Fernández, J. M., Villalón, M. & Verdugo, P. Biophys. J 59, 1022–1027 (1991).

    Article  Google Scholar 

  13. Curran, M. J. & Brodwick, M. S. J. gen. Physiol. 98, 771–790 (1991).

    Article  CAS  Google Scholar 

  14. Monck, J. R., Oberhauser, A. F., Alvarez de Toledo, G. & Fernandez, J. M. Biophys. J. 59, 39–47 (1991).

    Article  ADS  CAS  Google Scholar 

  15. Rabenstein, D. L. Biochemistry 26, 6923–6926 (1987).

    Article  CAS  Google Scholar 

  16. Daniels, A. J., Williams, R. P. J. & Wright, P. E. Neuroscience 3, 573–585 (1978).

    Article  CAS  Google Scholar 

  17. Almers, W., Breckenridge, L. J. & Spruce, A. E. Secretion and its Control (eds Oxford, G. & Armstrong, C.) 269–282 (The Rockefeller University Press, New York, 1989).

    Google Scholar 

  18. Heuser, J. E. & Reese, T. Handbook of Physiology Vol. 1 (ed. Kandel, E. R.) 261–294 (Williams & Wilkins, Bethesta, 1977).

    Google Scholar 

  19. Ludowyke, R. I. & Lagunoff, D. Biochemistry 25, 6287–6293 (1986).

    Article  CAS  Google Scholar 

  20. Millar, J. & Williams, G. V. J. electroanalyt. Chem. 282, 33–49 (1990).

    Article  CAS  Google Scholar 

  21. Joshi, C. & Fernández, J. M. Biophys. J. 53, 885–892 (1988).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Departamento de Fisiologia Medica y Biofisica, Facultad de Medicina, Universidad de Sevilla, Avda. Sanchez Pizjuan, 4 41009, Sevilla, Spain

    G. Alvarez de Toledo & R. Fernández-Chacón

  2. Department of Physiology & Biophysics, Mayo Clinic, Rochester, Minnesota, 55905, USA

    J. M. Fernández

Authors
  1. G. Alvarez de Toledo
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  2. R. Fernández-Chacón
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  3. J. M. Fernández
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de Toledo, G., Fernández-Chacón, R. & Fernández, J. Release of secretory products during transient vesicle fusion. Nature 363, 554–558 (1993). https://doi.org/10.1038/363554a0

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