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Amplification and sequencing of DNA from a 120–135-million-year-old weevil

Abstract

DNA has been successfully isolated from both fossilized plant1 and animal tissues2–6. The oldest material, dated as 25–40 million years old (Tertiary), was obtained from amber-entombed bees4,5 and termites6. Tissues from both these insects yielded DNA of good quality, which could be amplified by the polymerase chain reaction (PCR) and subsequently sequenced, including the genes encoding 18S ribosomal RNA5,6 and 16S rRNA6. We report here the extraction of DNA from a 120–135-million-year-old weevil (Nemonychidae, Coleoptera) found in Lebanese amber, PCR amplification of segments of the 18S rRNA gene and the internal transcribed spacer, and the corresponding nucleotide sequences of their 315- and 226-base-pair fragments, respectively. These sequen-ces were used for preliminary phylogenetic analysis of the nemonychid's sequence with three extant coleopterans: Lecontellus pinicola (Nemonychidae), Hypera brunneipennis (Curculionidae) and the mealworm Tenebrio molitor (Tenebrionidae), and two extant dipterans: the fruitfly Drosophila melanogaster (Drosophilidae) and mosquito Aedes albopictus (Culicidae) for the purpose of ascertaining the origin of the extracted and amplified DNA. The results revealed that the PCR-amplifted material is that of the extinct nemonychid weevil. This represents the oldest fossil DNA ever extracted and sequenced, extending by 80 million years the age of any previously reported DNA4–6.

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References

  1. Golenberg, E. M. et al. Nature 344, 656–658 (1990).

    Article  ADS  CAS  Google Scholar 

  2. Higuchi, R., Bowman, B., Freiberger, M., Ryder, O. A., & Wilson, A. C. Nature 312, 282–284 (1984).

    Article  ADS  CAS  Google Scholar 

  3. Thomas, R. H., Schaffner, W., Wilson, A. C. & Pääbo, S. Nature 340, 465–467 (1989).

    Article  ADS  CAS  Google Scholar 

  4. Cano, R. J., Poinar, H. N. & Poinar, G. O. Jr Med. Sci. Res. 20, 249–251 (1992).

    CAS  Google Scholar 

  5. Cano, R. J., Poinar, H. N. & Poinar, G. O. Jr Med. Sci. Res. 20, 619–623 (1992).

    CAS  Google Scholar 

  6. DeSalle, R., Gatesy, J., Wheeler, W. & Grimaldi, D. Science 257, 1860–1862 (1992).

    Article  Google Scholar 

  7. Poinar, J. G. O. Jr Life in Amber (Stanford Univ. Press, Stanford, 1992).

    Google Scholar 

  8. Schlee, D. & Dietrich, H.-G. Neues Jahbr. Geol. Paläont. Monatshefte (Stuttgart) 1, 40–50 (1970).

    Google Scholar 

  9. Schlee, W. & Glöckner, W. Bernestein 1–72 (Stuttgater Beitr, Naturk, Germany, 1978).

    Google Scholar 

  10. Kuschel, G. & Poinar, G. O. Jr A. Ent. Scand. (in the press).

  11. Crowson, R. A. The Biology of Coleotera (Academic, London, 1981).

    Google Scholar 

  12. Crowson, R. A. Entomol. Gener., Stuttgart 11, 57–58 (1985).

    Article  Google Scholar 

  13. Kuschel, G. Geogrl J. 7, 499–504 (1983).

    Google Scholar 

  14. Arnoldi, L. V. Trudy Paleont. Inst. 161, 142–176 (1977).

    Google Scholar 

  15. Bandel, K. & Vavra, N. N. Jb. Geol. Palecnt. Mk. 1, 19–33 (1981).

    Google Scholar 

  16. Whalley, P. Insects from Lebanese Amber (British Museum of Natural History (Geol.), London, 1981).

    Google Scholar 

  17. Crowson, R. A. Atti X Congr. Nazionale Italiano di Entomologia Sassari 1–90 (1974).

  18. Walsh, P. S., Metzger, D. A. & Higuchi, R. Bio Techniques 10, 506–513 (1991).

    CAS  Google Scholar 

  19. Jung, J. M., Comey, C. T., Baer, D. B. & Budowle, B. Int. J. Legal Med. 104, 145–148 (1991).

    Article  CAS  Google Scholar 

  20. Dams, E. et al. Nucleic Acids Res. 16 (suppl.), r87–rl73 (1988).

    Article  CAS  Google Scholar 

  21. White, T. J., Bruns, T., Lee, S. & Taylor, J. in PCR Protocols, a Guide to Methods and Applications (ed. Innes, M., Gelfand, D. H., Sninsky, J. J. & White, T. J.) 315–324 (Academic, New York, 1990).

    Google Scholar 

  22. Gyllensten, U. B. & Erlich, H. A. Proc. natn. Acad. Sci. U.S.A. 85, 7652–7656 (1988).

    Article  ADS  CAS  Google Scholar 

  23. Felsenstein, J. Cladistics 5, 164–166 (1989).

    Google Scholar 

  24. Swofford, D. Illinois Nat. Hist. Survey, Champaign, Illinois (1991).

  25. Saitou, N. & Nei, M. Molec. Biol. Evol. 4, 406–425 (1987).

    CAS  PubMed  Google Scholar 

  26. Hedges, S. B. Molec. Biol. Evol. 9, 366–369 (1992).

    CAS  PubMed  Google Scholar 

  27. Kimura, M. J. molec. Evol. 16, 111–120 (1980).

    Article  ADS  CAS  Google Scholar 

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Cano, R., Poinar, H., Pieniazek, N. et al. Amplification and sequencing of DNA from a 120–135-million-year-old weevil. Nature 363, 536–538 (1993). https://doi.org/10.1038/363536a0

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