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The mariner transposable element is widespread in insects

Nature volume 362, pages 241–245 (1993)Cite this article

Abstract

THE mariner transposable element is a small member of the short inverted terminal repeat class thought to transpose through a DNA intermediate1. Originally described in Drosophila mauritiana2, it is now known in several species of the family Drosophilidae3,4, and in a moth Hyalophora cecropia5. Here I use primers designed to represent regions of amino-acid conservation between the putative transposase genes of the D. mauritiana and H. cecropia elements to amplify equivalent regions of presumed mariner elements from ten other insects representing six additional orders, including the malaria-vector mosquito, Anopheles gambiae. Sequences of multiple clones from each species reveal a diverse array of mariner elements, with multiple subfamilies in the genomes of some insects, indicating both vertical inheritance and horizontal transfers. An intact open reading frame in at least one clone from each species suggests each may carry functional transposable elements. Therefore the mariner element is an excellent candidate for development of genetic transformation systems for non-drosophilid insects, and possibly other arthropods.

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References

  1. Hartl, D. L. in Mobile DNA (eds Berg, D. E. & Howe, M. M.) 5531–5536 (Am. Soc. Microbiol., Washington DC, 1989).

    Google Scholar 

  2. Jacobson, J. W., Medhora, M. M. & Hartl, D. L. Proc. natn. Acad. Sci. U.S.A. 83, 8684–8688 (1986).

    Article  ADS  CAS  Google Scholar 

  3. Maruyama, K. & Hartl, D. L. Genetics 128, 319–329 (1991).

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Maruyama, K. & Hartl, D. L. J. molec. Evol. 33, 514–524 (1991).

    Article  ADS  CAS  Google Scholar 

  5. Lidholm, D.-A., Gudmundsson, G. H. & Boman, H. G. J. biol. Chem. 266, 11518–11521 (1991).

    CAS  PubMed  Google Scholar 

  6. Pearson, W. R. & Lipman, D. J. Proc. natn. Acad. Sci. U.S.A. 85, 2444–2448 (1988).

    Article  ADS  CAS  Google Scholar 

  7. Swofford, D. I. PALP: Phylogenetic Analysis Using Parsimony, Version 3 (Illinois Natural History Survey, Champaign, Illinois, 1991).

    Google Scholar 

  8. Medhora, M., Maruyama, K. & Hartl, D. L. Genetics 128, 311–318 (1991).

    CAS  PubMed  PubMed Central  Google Scholar 

  9. Eckert, K. A. & Kunkel, T. A. PCR Meth. Appl. 1, 17–24 (1991).

    Article  CAS  Google Scholar 

  10. Kristensen, N. P. in The Insects of Australia 2nd edn 125–140 (CSIRO, Melbourne University Press, Australia, 1991).

    Google Scholar 

  11. Hennig, W. Insect Phylogeny (Wiley, New York, 1981).

    Google Scholar 

  12. Carpenter, F. M. Psyche 83, 336–376 (1976).

    Article  Google Scholar 

  13. Simonelig, M. & Anxolabéhères, D. Proc. natn. Acad. Sci. U.S.A. 88, 6102–6106 (1991).

    Article  ADS  CAS  Google Scholar 

  14. Daniels, S. B., Petersen, K. R., Strausbaugh, L. D., Kidwell, M. G. & Chovnick, A. Genetics 129, 399–355 (1990).

    Google Scholar 

  15. O'Neill, S. L. Giordano, R., Colbert, A. M. E., Karr, T. L. & Robertson, H. M. Proc. natn. Acad. Sci. U.S.A. 89, 2699–2702 (1992).

    Article  ADS  CAS  Google Scholar 

  16. Houck, M. A., Clark, J. B., Peterson, K. R. & Kidwell, M. G. Science 253, 1125–1128 (1991).

    Article  ADS  CAS  Google Scholar 

  17. Hurst, G. D. D., Hurst, L. D. & Majerus, M. E. N. Nature 356, 659–660 (1992).

    Article  ADS  CAS  Google Scholar 

  18. Garza, D., Medhora, M., Koga, A. & Hartl, D. L. Genetics 128, 303–310 (1991).

    CAS  PubMed  PubMed Central  Google Scholar 

  19. Saiki, R. K. et al. Science 239, 487–491 (1988).

    Article  ADS  CAS  Google Scholar 

  20. Qian, L. & Wilkinson, M. BioTechniques 10, 736–738 (1991).

    CAS  PubMed  Google Scholar 

  21. Holton, T. A. & Graham, M. W. Nucleic Acids Res. 19, 1156 (1991).

    Article  CAS  Google Scholar 

  22. Holmes, D. S. & Quigley, M. Analyt. Biochem. 114, 193 (1981).

    Article  CAS  Google Scholar 

  23. Sambrook, J., Fritsch, E. F. & Maniatis, T. Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, New York, 1989).

    Google Scholar 

  24. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  25. Higgins, D. G. & Sharp, P. M. CABIOS 5, 151–153 (1989).

    CAS  Google Scholar 

Download references

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  1. Department of Entomology, University of Illinois, 505 South Goodwin Avenue, Urbana, Illinois, 61801, USA

    Hugh M. Robertson

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  1. Hugh M. Robertson
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Robertson, H. The mariner transposable element is widespread in insects. Nature 362, 241–245 (1993). https://doi.org/10.1038/362241a0

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