Abstract
THE centrifugal elongation of membranes to form extended tubular structures is a widespread form of intracellular organelle movement. Tubular lysosomes1 and the endoplasmic reticulum2, for example, undergo such extension in association with micro-tubules, and this process has been mimicked in vitro by combining purified microtubules with isolated membranes and the mechano-chemical ATPase kinesin3,4. This, along with evidence that kinesin is associated with the endoplasmic reticulum5, has led to the suggestion that kinesin provides the motive force for the formation and maintenance of elongated tubulovesicular structures in cells6,7. We have addressed this hypothesis in murine macrophages, which have prominent tubular lysosomes whose form depends on the integrity of microtubules. Here we report that two antikinesin antibodies which disrupt in vitro motility will each cause centripetal collapse of the array of tubular lysosomes when scrape-loaded into macrophages. To our knowledge this provides the first in vivo evidence that kinesin is responsible for extension of tubulovesicular structures along microtubules.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Swanson, J. A., Bushnell, A. & Silverstein, S. C. Proc. natn. Acad. Sci. U.S.A. 84, 1921–1925 (1987).
Lee, C. & Chen, L. B. Cell 54, 37–46 (1988).
Dabora, S. L. & Sheetz, M. P. Cell 54, 27–35 (1988).
Vale, R. D. & Hotani, H. J. Cell Biol. 107, 2233–2241 (1988).
Hollenbeck, P. J. J. Cell Blol. 108, 2335–2342 (1989).
Sheetz, M. P. Bioessays 7, 165–168 (1987).
Vale, R. D. A. Rev. Cell Biol. 3, 347–378 (1987).
Herman, B. & Albertini, D. F. J. Cell Biol. 98, 565–576 (1984).
Matteoni, R. & Kreis, T. E. J. Cell biol. 105, 1253–1265 (1987).
Vale, R. D., Reese, T. S. & Sheetz, M. P. Cell 42, 39–50 (1985).
Brady, S. T. Nature 317, 73–75 (1985).
Hollenbeck, P. J. Protoplasms 145, 145–152 (1988).
Vale, R. D. et al. Cell 43, 623–632 (1985).
Ingold, A. L., Cohn, S. A. & Scholey, J. M. J. Cell Biol. 107, 2657–2667 (1988).
Scholey, J. M., Heuser, J., Yang, J. T. & Goldstein, L. S. B. Nature 338, 355–357 (1989).
McNeil, P. L., Murphy, R. F., Lanni, F. & Taylor, D. L. J. Cell Biol. 98, 1556–1564 (1984).
Pfister, K. K., Wagner, M. C., Stenoien, D. L., Brady, S. T. & Bloom, G. S. J. Cell Biol. 108, 1453–1463 (1989).
Schroer, T. A., Schnapp, B. J., Reese, T. S. & Sheetz, M. P. J. Cell Biol. 107, 1785–1792 (1988).
Swanson, J. A. J. Cell Sci. 94, 135–142 (1989).
Hollenbeck, P. J., Suprynowicz, F. & Cande, W. Z. J. Cell Biol. 99, 1251–1258 (1984).
Wessel, D. & Flügge, U, I. Analyst Biochem. 138, 141–143 (1984).
Bruck, C., Portetelle, D., Glineur, C. & Bollen, A. J. immunol. Meth. 53, 313–319 (1982).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hollenbeck, P., Swanson, J. Radial extension of macrophage tubular lysosomes supported by kinesin. Nature 346, 864–866 (1990). https://doi.org/10.1038/346864a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/346864a0
This article is cited by
-
RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin
Nature Communications (2022)
-
The role of lysosomes in cancer development and progression
Cell & Bioscience (2020)
-
2-Hydroxypropyl-gamma-cyclodextrin overcomes NPC1 deficiency by enhancing lysosome-ER association and autophagy
Scientific Reports (2020)
-
Autophagic and endo-lysosomal dysfunction in neurodegenerative disease
Molecular Brain (2019)
-
MHC class II molecules on the move for successful antigen presentation
The EMBO Journal (2008)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.