Mycoplasma contamination is tough to detect and even more difficult to eradicate. It is best to start over fresh from clean cell stocks, but several elimination options are available.
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Amyloid-beta peptide degradation in cell cultures by mycoplasma contaminants
BMC Research Notes Open Access 30 June 2008
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References
McGarrity, G.J. Adv. Cell Culture 2, 99–131 (1982).
DelGuidice, R.A. & Gardella, R.S. Tissue Culture Association Monograph 5, 104–115 (US Tissue Culture Association, Gaithersburg, Maryland, 1984).
Chen, T.R. Expl Cell Res. 104, 255–263 (1977).
Russell, W.C., Newman, C. & Williamson, D.H. Nature 253, 461–462 (1975).
Barile, M.F. & Grabowski, M.W. Meth. Mycoplasmol. 2, 173–181 (1983).
McGarrity, G.J. & Carson, D.A. Expl Cell Res. 139, 199–205 (1982).
Gabridge, M.E., Lundin, D.J. & Gladd, M.F. In Vitro Cell Dev. Biol. 22, 491–498 (1986).
Hay, R.J. Analyt. Biochem. 171, 225–237 (1988).
Jeansson, S. & Brorson, J.K. Expl Cell Res. 161, 181–188 (1985).
Van Diggelen, O.P., Shin, S. & Phillips, D.M. Cancer Res. 37, 2680–2687 (1977).
Schimmelpfeng, L., Langenberg, U. & Peters, H.J. Nature 285, 661–662 (1980).
Marcus, M., Lavi, U., Nattenberg, A., Rottem, S., & Markowitz, O. Nature 285, 659–661 (1980).
Hay, R.J. Prog. Cancer Res. Ther. 29, 3–21 (1984).
Borup-Christensen, P., Erb, K. & Jensen, J.C. J. Immunol. Meth. 110, 237–240 (1988).
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Hay, R., Macy, M. & Chen, T. Mycoplasma infection of cultured cells. Nature 339, 487–488 (1989). https://doi.org/10.1038/339487a0
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DOI: https://doi.org/10.1038/339487a0
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