Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Induction of proto-oncogene JUN/AP-1 by serum and TPA

Nature volume 334, pages 629–631 (1988)Cite this article

Abstract

The response of a cell to mitogens and differentiation agents involves the transcriptional induction of several cellular genes. Prominent among these so-called 'immediate early' or 'competence' genes are the nuclear oncogenes fos and mycl–7. Although the precise function of these early response genes in growth control is not understood, it is likely that many of them are involved in the transition from G0 to G1 in the cell cycle. The findings that the products of nuclear proto-oncogenes jun and erbA are transcriptional factors supports the notion of the role of the nuclear oncoproteins in the regulation of gene expression8–11. Recently, it has been reported that the FOS protein is associated in transcriptional complexes with the product of the jun oncogene, the transcription factor AP-1 (refs 12–15). As the fos gene is induced in response to mitogens during initiation of cell growth, we investigated whether expression of the nuclear transcription factor AP-1 is also inducible. We report that mouse c-jun gene transcription is rapidly induced by serum and phorbol-ester 12-o-tetradecanoyl phorbol 13-acetate (TPA). Furthermore, induction is transient and the mRNA is superinduced by inhibitors of protein synthesis.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Cochran, B. H., Reffel, A. C. & Stiles, C. D. Cell 33, 939–947 (1983).

    Article  CAS  Google Scholar 

  2. Kelly, K., Cochran, B. H., Stiles, C. D. & Leder, P. Cell 35, 603–610 (1983).

    Article  CAS  Google Scholar 

  3. Greenberg, M. E. & Ziff, E. B. Nature 311, 433–438 (1984).

    Article  ADS  CAS  Google Scholar 

  4. Kruijer, W., Cooper, J. A., Hunter, T. & Verma, I. M. Nature 312, 711–716 (1984).

    Article  ADS  CAS  Google Scholar 

  5. Muller, R., Bravo, R., Burckhardt, J. & Curran, T. Nature 312, 716–720 (1984).

    Article  ADS  CAS  Google Scholar 

  6. Lau, L. F. & Nathans, D. Proc. natn. Acad. Sci. U.S.A. 84, 1182–1186 (1987).

    Article  ADS  CAS  Google Scholar 

  7. Almendral, J. M. et al. Malec. cell. Biol. 8, 2140–2148 (1988).

    CAS  Google Scholar 

  8. Bohmann, D. et al. Science 238, 1386–1392 (1987).

    Article  ADS  CAS  Google Scholar 

  9. Angel, P. et al. Nature 332, 166–171 (1988).

    Article  ADS  CAS  Google Scholar 

  10. Sap, J. et al. Nature 324, 635–640 (1986).

    Article  ADS  CAS  Google Scholar 

  11. Weinberger, C. et al. Nature 324, 641–646 (1986).

    Article  ADS  CAS  Google Scholar 

  12. Rauscher, F. J. III, Sambucetti, L. C., Curran, T., Distel, R. J. & Spiegelman, B. M. Cell 52, 471–480 (1988).

    Article  CAS  Google Scholar 

  13. Franza, R. B., Jr., Rauscher, F. J. III, Josephs, S. F. & Curran, T. Science 239, 1150–1153 (1988).

    Article  ADS  CAS  Google Scholar 

  14. Rauscher, F. J. III et al. Science 240, 1010–1016 (1988).

    Article  ADS  CAS  Google Scholar 

  15. Sassone-Corsi, P., Lamph, W. W., Kamps, M. & Verma, I. M. Cell 54 (in the press).

  16. Lee, W., Mitchell, P. & Tjian, R. Cell 49, 741–752 (1987).

    Article  CAS  Google Scholar 

  17. Angel, P. et al. Cell 49, 729–739 (1987).

    Article  CAS  Google Scholar 

  18. Ryder, K., Lau, L. F. & Nathans, D. Proc. natn. Acad. Sci. U.S.A. 85, 1487–1491 (1988).

    Article  ADS  CAS  Google Scholar 

  19. Vogt, P. K., Bos, T. J. & Doolittle, R. F. Proc. natn. Acad. Sci. U.S.A. 84, 3316–3319 (1987).

    Article  ADS  CAS  Google Scholar 

  20. Shaw, G. & Kamen, R. Cell 46, 659–667 (1986).

    Article  CAS  Google Scholar 

  21. Curran, T., Miller, A. D., Zokas, L. & Verma, I. M. Cell 36, 259–268 (1984).

    Article  CAS  Google Scholar 

  22. Barber, J. R. & Verma, I. M. Molec. cell. Biol. 7, 2201–2211 (1987).

    Article  CAS  Google Scholar 

  23. Kryszke, M.-H., Piette, J. & Yaniv, M. Nature 328, 254–256 (1987).

    Article  ADS  CAS  Google Scholar 

  24. Devereux, J., Haeberli, P. & Smithies, O. Nucleic Acids Res. 12(1), 387–195 (1984).

    Article  CAS  Google Scholar 

  25. Okayama, H. & Berg, P. Molec. cell. Biol. 3, 280–289 (1983).

    Article  CAS  Google Scholar 

  26. Maniatis, T., Fritsch, E. F. & Sambrook, J. Molecular Cloning (Cold Spring Harbor Laboratory, 1982).

    Google Scholar 

  27. Henikoff, S. Gene 28, 351–359 (1984).

    Article  CAS  Google Scholar 

  28. Sanger, F. et al. Nature 265, 687–695 (177).

  29. Mitchell, R. L., Zokas, L., Schreiber, R. D. & Verma, I. M. Cell 40, 209–217 (1985).

    Article  CAS  Google Scholar 

  30. Sassone-Corsi, P. & Verma, I. M. Nature 326, 507–510 (1987).

    Article  ADS  CAS  Google Scholar 

  31. Gorman, C. M., Moffat, L. F. & Howard, B. H. Molec. cell. Biol. 2, 1044–1051 (1982).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Molecular Biology and Virology Laboratory, The Salk Institute, PO Box 85800, San Diego, California, 92138, USA

    William W. Lamph, Penny Wamsley, Paolo Sassone-Corsi & Inder M. Verma

Authors
  1. William W. Lamph
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Penny Wamsley
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Paolo Sassone-Corsi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Inder M. Verma
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lamph, W., Wamsley, P., Sassone-Corsi, P. et al. Induction of proto-oncogene JUN/AP-1 by serum and TPA. Nature 334, 629–631 (1988). https://doi.org/10.1038/334629a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/334629a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing