Nature Publishing Group, publisher of Nature, and other science journals and reference works
Nature
my account e-alerts subscribe register
   
Tuesday 23 May 2017
Journal Home
Current Issue
AOP
Archive
Download PDF
References
Export citation
Export references
Send to a friend
More articles like this

Letters to Nature
Nature 333, 664 - 666 (16 June 1988); doi:10.1038/333664a0

Vascular endothelial cells synthesize nitric oxide from L-arginine

R. M. J. Palmer, D. S. Ashton & S. Moncada*

Wellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS, UK
*To whom correspondence should be addressed.

Nitric oxide (NO) released by vascular endothelial cells accounts for the relaxation of strips of vascular tissue1 and for the inhibition of platelet aggregation2 and platelet adhesion3 attributed to endothelium-derived relaxing factor4. We now demonstrate that NO can be synthesized from L-arginine by porcine aortic endothelial cells in culture. Nitric oxide was detected by bioassay5, chemiluminescence1 or by mass spectrometry. Release of NO from the endothelial cells induced by bradykinin and the calcium ionophore A23187 was reversibly enhanced by infusions of L-arginine and L-citrulline, but not D-arginine or other close structural analogues. Mass spectrometry studies using 15N-labelled L-arginine indicated that this enhancement was due to the formation of NO from the terminal guanidino nitrogen atom(s) of L-arginine. The strict substrate specificity of this reaction suggests that L-arginine is the precursor for NO synthesis in vascular endothelial cells.

------------------

References

1. Palmer, R. M. J., Ferrige, A. G. & Moncada, S. Nature 327, 524−526 (1987). | Article | PubMed | ISI | ChemPort |
2. Radomski, M. W., Palmer, R. M. J. & Moncada, S. Br. J. Pharmac. 92, 639−646 (1987). | ChemPort |
3. Radomski, M. W., Palmer, R. M. J. & Moncada, S. Biochem. biophys. Res. Commun. 148, 1482−1489 (1987). | Article | PubMed | ISI | ChemPort |
4. Furchgott, R. F. & Zawadzki, J. V. Nature 288, 373−376 (1980). | Article | PubMed | ISI | ChemPort |
5. Gryglewski, R. J., Moncada, S. & Palmer, R. M. J. Br. J. Pharmac. 87, 685−694 (1986). | ISI | ChemPort |
6. Salmon, J. A. Prostaglandins 15, 383−397 (1978). | Article | PubMed | ChemPort |
7. Gryglewski, R. J., Palmer, R. M. J. & Moncada, S. Nature 320, 454−456 (1986). | Article | PubMed | ISI | ChemPort |
8. Rubanyi, G. M. & Vanhoutte, P. M. Am. J. Physiol. 250, H222−H227 (1986).
9. Stuehr, D. J. & Marietta, M. A. Proc. natn. Acad. Sci. U.S.A. 82, 7738−7742 (1985). | ChemPort |
10. Hibbs, J. B., Taintor, R. R. & Vavrin, Z. Science 235, 473−476 (1987). | PubMed | ISI | ChemPort |
11. Hibbs, J. B., Vavrin, Z. & Taintor, R. R. J. Immun. 136, 550−565 (1987).
12. Iyengar, R., Stuehr, D. J. & Marietta, M. A. Proc. natn. Acad. Sci. U.S.A. 84, 6369−6373 (1987). | ChemPort |



© 1988 Nature Publishing Group
Privacy Policy