Abstract
A yeast cytosol is shown to contain two distinct activities that stimulate protein translocation across microsomal membranes. One activity was purified. It consists of two constitutively expressed 70K heat shock related proteins that increase the rate of translocation. Possible mechanisms of action of these proteins are discussed.
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References
Walter, P. & Lingappa, V. R. A. Rev. Cell Biol. 2, 499–516 (1986).
Waters, M. G. & Blobel, G. J. Cell Biol. 102, 1543–1550 (1986).
Hansen, W., Garcia, P. D. & Walter, P. Cell 45, 397–406 (1986).
Rothblatt, J. A. & Meyer, D.I. Cell 44, 629–637 (1986).
Addison, R. J. biol. Chem. 262, 17031–17037 (1987).
Waters, M. G., Chirico, W. J. & Blobel, G. J. Cell Biol. 103, 2629–2636 (1986).
Ingolia, T. D., Slater, M. R. & Craig, E. A. Molec. Cell Biol. 2, 1388–1398 (1982).
Craig, E. A. CRC Crit. Rev. Biochem. 18, 239–280 (1985).
Kurjan, J. & Herskonwitz, I. Cell 30, 933–943 (1982).
Sprague, Jr, G. F., Blair, L. & Thorner, J. A. Rev. Microbiol. 37, 623–660 (1983).
Waters, M. G., Evans, E. & Blobel, G. J. biol. Chem. (in the press).
Deshaies, R., Koch, B. D., Werner-Washburne, M., Craig, E. & Schekman, R. Nature (in the press).
Yue, V. T. & Schimmel, P. R. Biochemistry 16, 4678–4684 (1977).
Lindquist, S. A. Rev. Biochem. 55, 1151–1191 (1986).
Kurtz, S., Rossi, J., Petko, L. & Lindquist, S. Science 231, 1154–1157 (1982).
Welch, W. J. & Feramisco, J. R. J. biol. Chem. 257, 14949–14959.
Chappell, T. G., Welch, W. J., Schlossman, D. M., Palter, K. B., Schlesinger, M. J. & Rothman, J. E. Cell 45, 3–13 (1986).
Craig, E. A. & Jacobsen, K. Cell 38, 841–849 (1984).
Schlossman, D. M., Schmid, S. L., Braell, W. A. & Rothman, J. E. J. Cell Biol. 99, 723–733 (1984).
Pelham, H. R. B. Cell 46, 959–961 (1986).
Eilers, M. & Schatz, G. Nature 322, 228–232 (1986).
Randall, L. L. & Hardy, S. J. S. Cell 46, 921–928 (1986).
Walter, P. & Blobel, G. Nature 299, 691–698 (1982).
Wiedmann, M., Kurschalia, T. V., Hartmann, E. & Rapoport, T. A. Nature 328, 830–833 (1987).
Bradford, M. M. Analyt. Biochem. 72, 248–254 (1976).
Hicks, J. B. & Herskowitz, I. Genetics 83, 245–258 (1976).
Rothman, J. E. & Kornberg, R. D. Nature 322, 209–210 (1986).
Pfanner, N., Tropschug, M. & Neupert, W. Cell 49, 815–823 (1987).
Crooke, E. & Wickner, W. Proc. natn. Acad. Sci. U.S.A 84, 5216–5220 (1987).
Chen, W. & Douglas, M. G. J. biol. Chem. 262, 15598–15604 (1987).
Guidon, P. T. Jr, & Hightower, L. E. Biochemistry 25, 3231–3239 (1986).
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Chirico, W., Waters, M. & Blobel, G. 70K heat shock related proteins stimulate protein translocation into microsomes. Nature 332, 805–810 (1988). https://doi.org/10.1038/332805a0
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DOI: https://doi.org/10.1038/332805a0
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