Abstract
Human immunoglobulin chains are expressed in somatic cell hybrids between mouse myeloma cells and human B cells1,2. By correlating the expression of human immunoglobulin chains and the presence of specific human chromosomes in the hybrid clones, we have assigned the human immunoglobulin heavy-chain gene cluster to human chromosome 14 (refs 1,2). We have now studied somatic cell hybrids between mouse myeloma cells and either human peripheral lymphocytes or different human λ chain-secreting lymphoblastoid cell lines. Somatic cell hybrid clones were pre-selected for their ability to produce human λ immunoglobulin chains, and studied for the expression of isozyme markers assigned to each of the different human chromosomes. Subclones of human λ chain-secreting hybrid clones were also studied. The results indicate that human chromosome 22 carries the human λ immunoglobulin chain genes.
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Erikson, J., Martinis, J. & Croce, C. Assignment of the genes for human λ immunoglobulin chains to chromosome 22. Nature 294, 173–175 (1981). https://doi.org/10.1038/294173a0
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DOI: https://doi.org/10.1038/294173a0
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