Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Molecular cloning and sequencing of cDNA encoding the precursor to the small subunit of chloroplast ribulose-1,5-bisphosphate carboxylase

Nature volume 287, pages 692–697 (1980)Cite this article

Abstract

Polyadenylated RNA from leaves of pea (Pisum sativum) has been copied into DNA and cloned in the Escherichia coli plasmid pBR322. From these clones we have identified and sequenced DNA encoding the mRNA for the precursor of the small subunit of the chloroplast enzyme ribulose-1,5-bisphosphate carboxylase.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Ellis, R. J., Smith, S. M. & Barraclough, B. R. in Genome Organisation and Expression in Plants (ed. Leaver, C. J.) 321–335 (Plenum, New York, 1980).

    Book  Google Scholar 

  2. Chua, N-H. & Schmidt, G. W. J. Cell Biol. 81, 461–483 (1979).

    Article  CAS  Google Scholar 

  3. Ellis, R. J. Trends biochem. Sci. 4, 241–244 (1979).

    Article  CAS  Google Scholar 

  4. Jensen, R. G. & Bahr, J. T. A. Rev. Pl. Physiol. 28, 379–400 (1977).

    Article  CAS  Google Scholar 

  5. Coen, D. M., Bedbrook, J. R., Bogorad, L. & Rich, A. Proc. natn. Acad. Sci. U.S.A. 74, 5487–5491 (1977).

    Article  ADS  CAS  Google Scholar 

  6. Bedbrook, J. R., Coen, D. M., Beaton, A. R., Bogorad, L. & Rich, A. J. biol. Chem. 254, 905–910 (1977).

    Google Scholar 

  7. Blair, G. E. & Ellis, R. J. Biochim. biophys. Acta 319, 223–234 (1973).

    Article  CAS  Google Scholar 

  8. Kawashima, N. & Wildman, S. G. Biochim. biophys. Acta 262, 42–49 (1972).

    Article  CAS  Google Scholar 

  9. Dobberstein, B., Blobel, G. & Chua, N. H. Proc. natn. Acad. Sci. U.S.A. 74, 1082–1085 (1977).

    Article  ADS  CAS  Google Scholar 

  10. Highfield, P. E. & Ellis, R. J. Nature 271, 420–424 (1978).

    Article  ADS  CAS  Google Scholar 

  11. Chua, N. H. & Schmidt, G. W. Proc. natn. Acad. Sci. U.S.A. 75, 6110–6114 (1978).

    Article  ADS  CAS  Google Scholar 

  12. Smith, S. M. & Ellis, R. J. Nature 278, 662–664 (1979).

    Article  ADS  CAS  Google Scholar 

  13. Blobel, G. & Dobberstein, B. J. Cell Biol. 67, 835–851 (1975).

    Article  CAS  Google Scholar 

  14. Link, G., Coen, D. M. & Bogorad, L. Cell 15, 725–731 (1978).

    Article  CAS  Google Scholar 

  15. Grunstein, M. & Hogness, D. S. Proc. natn. Acad. Sci. U.S.A. 72, 3961–3965 (1975).

    Article  ADS  CAS  Google Scholar 

  16. Smith, D. F., Searle, P. F. & Williams, J. G. Nucleic Acids Res. 6, 487–506 (1979).

    Article  CAS  Google Scholar 

  17. Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

    Article  ADS  CAS  Google Scholar 

  18. Bedbrook, J. & Bogorad, L. Proc. natn. Acad. Sci. U.S.A. 73, 4309–4313 (1976).

    Article  ADS  CAS  Google Scholar 

  19. Smith, H. O. & Birnstiel, M. L. Nucleic Acids Res. 3, 2387–2398 (1976).

    Article  CAS  Google Scholar 

  20. Martin, P. G. Aust. J. Pl. Physiol. 6, 401–408 (1979).

    CAS  Google Scholar 

  21. Schmidt, G. W., Devilliers-Thiery, A., Desruisseaux, H., Blobel, G. & Chua, N.-H. J. Cell Biol. 83, 615–622 (1979).

    Article  CAS  Google Scholar 

  22. Cheng, C. C. et al. J. molec. Biol. 107, 527–547 (1976).

    Article  CAS  Google Scholar 

  23. McMaster, G. K. & Carmichael, G. G. Proc. natn. Acad. Sci. U.S.A. 74, 4835–4838 (1977).

    Article  ADS  CAS  Google Scholar 

  24. Alwine, J. C., Kemp, D. J. & Stark, G. R. Proc. natn. Acad. Sci. U.S.A. 74, 5350–5354 (1977).

    Article  ADS  CAS  Google Scholar 

  25. Austen, B. M. FEBS Lett. 103, 308–313 (1977).

    Article  Google Scholar 

  26. Loening, U. E. Biochem. J. 113, 131–138 (1969).

    Article  CAS  Google Scholar 

  27. Myers, J. C. & Spiegelman, S. Proc. natn. Acad. Sci. U.S.A. 75, 5329–5333 (1978).

    Article  ADS  CAS  Google Scholar 

  28. Boyer, H. W. et al. in Recombinant Molecules; Impact on Science and Society (eds Beers, R. F. & Bassett, E. G.) (Raven, New York, 1977).

    Google Scholar 

  29. Boyer, H. W. & Roulland-Dossaix, D. J. molec. Biol. 41, 459–472 (1969).

    Article  CAS  Google Scholar 

  30. Roychoudhury, R., Jay, E. & Wu, R. Nucleic Acids Res. 3, 101–116 (1976).

    Article  CAS  Google Scholar 

  31. Maizels, N. Cell 9, 431–438 (1976).

    Article  CAS  Google Scholar 

  32. Clewell, D. B. J. Bact. 110, 667–676 (1972).

    CAS  Google Scholar 

  33. Dingman, C. W. & Peacock, A. C. Biochemistry 7, 659–668 (1968).

    Article  CAS  Google Scholar 

  34. Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Plant Breeding Institute, Maris Lane, Trumpington, Cambridge, CB2 2LQ

    John R. Bedbrook

  2. UK Division of Plant Industry, CSIRO, PO Box 1600, Canberra City, ACT, 2601, Australia

    John R. Bedbrook

  3. Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK

    Steven M. Smith & R. John Ellis

Authors
  1. John R. Bedbrook
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Steven M. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. John Ellis
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bedbrook, J., Smith, S. & Ellis, R. Molecular cloning and sequencing of cDNA encoding the precursor to the small subunit of chloroplast ribulose-1,5-bisphosphate carboxylase. Nature 287, 692–697 (1980). https://doi.org/10.1038/287692a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/287692a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing