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Sequence of the lactose permease gene

Nature volume 283, pages 541–545 (1980)Cite this article

Abstract

The nucleotide sequence of the lacY gene coding for lactose permease (M protein) in Escherichia coli has been determined. The sequence includes the intergenic regions between the lacZ (β-galactosidase) and lacY genes as well as the region between the lacY and lacA (transacetylase) genes. Lactose permease is predicted to consist of 417 residues (71% nonpolar), resulting in a protein with a molecular weight of 46,504. The reading frame was confirmed by the sequence of a nonsense mutation changing codon 33 from UGG to UAG.

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Authors and Affiliations

  1. Institute for Genetics, University of Cologne, Weyertal 121, D-5000, Cologne, 41, FRG

    Dagmar E. Büchel, Bruno Gronenborn & Benno Müller-Hill

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  1. Dagmar E. Büchel
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  2. Bruno Gronenborn
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  3. Benno Müller-Hill
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Büchel, D., Gronenborn, B. & Müller-Hill, B. Sequence of the lactose permease gene. Nature 283, 541–545 (1980). https://doi.org/10.1038/283541a0

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