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Nature 275, 617-624 (19 October 1978) | doi:10.1038/275617a0; Accepted 15 September 1978

Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase

Annie C. Y. Chang*, †, §, Jack H. Nunberg‡, Randal J. Kaufmanparallel, Henry A. Erlich†, Robert T. Schimke‡ & Stanley N. Cohen*, †, 

  1. Departments of Genetics*, , Medicine†, , Biological Sciences‡, , Medical Microbiology§, , and Pharmacologyparallel, Stanford University, Stanford, California 94305
  2. To whom correspondence should be addressed at Room S337, Stanford University Medical Center, Stanford, California 94305.
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The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the anti-metabolic drug trimethoprim.