Abstract
The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the anti-metabolic drug trimethoprim.
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Chang, A., Nunberg, J., Kaufman, R. et al. Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase. Nature 275, 617–624 (1978). https://doi.org/10.1038/275617a0
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DOI: https://doi.org/10.1038/275617a0
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