Abstract
Blunt end ligation with an EcoRI/ octanucleotide “linker” was used to construct a plasmid containing chemically synthesized lac operator DNA. This plasmid renders the host bacterium constitutive for β-galactosidase.
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Heyneker, H., Shine, J., Goodman, H. et al. Synthetic lacoperator DNA is functional in vivo. Nature 263, 748–752 (1976). https://doi.org/10.1038/263748a0
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DOI: https://doi.org/10.1038/263748a0
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