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Chromosome Breakage by Deoxyribonuclease

Nature volume 227, pages 707–708 (1970)Cite this article

Abstract

THE possibility that endogenous deoxyribonuclease might play a part in inducing chromatid breakage was suggested by our experiments describing a high incidence of chromosome breaks and rearrangements in human diploid cell strains after selective damage to lysosomes1. We describe here a similar effect when exogenous deoxyribonuclease is introduced into cells in the presence of hypertonic MgSO4. This is a modification of the method used by Wecker2 in assays of the infectivity of extracted viral nucleic acid.

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Authors and Affiliations

  1. Clinical Research Centre Laboratories, National Institute for Medical Research, Mill Hill, London, NW7

    GILLIAN R. PATON & A. C. ALLISON

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  1. GILLIAN R. PATON
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  2. A. C. ALLISON
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PATON, G., ALLISON, A. Chromosome Breakage by Deoxyribonuclease. Nature 227, 707–708 (1970). https://doi.org/10.1038/227707a0

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