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Isolation and Purification of Intact Gas Vesicles from a Blue–Green Alga

Nature volume 224, pages 716–717 (1969)Cite this article

Abstract

THE gas vesicles which make up the gas vacuoles of blue–green algae collapse flat on application of a few atmospheres pressure1 and this frustrates attempts to isolate them intact by methods of cell rupture and centrifugation used in obtaining preparations of other subcellular organelles2. We describe here a novel method of lysing blue–green algal cells, the isolation of their intact gas vesicles and a preliminary investigation into the composition of the gas vesicle membrane.

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Authors and Affiliations

  1. Department of Botany, Westfield College, London, NW3

    A. E. WALSBY & BARBARA BUCKLAND

Authors
  1. A. E. WALSBY
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  2. BARBARA BUCKLAND
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WALSBY, A., BUCKLAND, B. Isolation and Purification of Intact Gas Vesicles from a Blue–Green Alga. Nature 224, 716–717 (1969). https://doi.org/10.1038/224716a0

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