Abstract
VOLUNTEERS were injected with conjugates of 2,4-dinitro-phenyl–human serum albumin (DNP–HSA)1. Sixty per cent of subjects developed rising titres of agglutinating activity to DNP coupled red cells after injection, but most normal pre-immunization sera also had some agglutinating activity to DNP coupled cells. This activity was present in the fractions containing gamma globulin, was abolished by 2-mercaptoethanol, was specifically inhibited by the DNP haptenic determinant and was deficient in sera that were deficient in normal immunoglobulins—suggesting that most normal human sera contain antibody to the DNP group.
Similar content being viewed by others
Article PDF
References
Brandriss, M. W., and Bullock, W. E., Fed. Proc., 27, 263 (1968).
Bullock, W. E., and Kantor, F. S., J. Immunol., 94, 317 (1965).
Fahey, J. L., and McKelvey, E. M., J. Immunol., 94, 84 (1965).
Cheng, W. C., and Talmage, D. W., J. Immunol., 97, 778 (1966).
Campbell, D. M., Garvey, J. S., Cremer, N. E., and Sussdorf, D. M., Methods in Immunology, 79 (Benjamin, W. A., Inc., New York, 1963).
Brandriss, M. W., Smith, J. W., and Steinman, H. G., J. Immunol., 94, 696 (1965).
Eisen, H. N., Little, J. R., Osterland, C. K., and Simms, E. S., Cold Spring Harbor Symp. Quant. Biol., 32, 75 (1967).
Boyden, S. V., in Advances in Immunology, 5 (edit. by Dixon, jun., F. J., and Humphrey, J. H.) (Academic Press, New York, 1966).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
BRANDRISS, M. Antibody-like Activity to the 2,4-Dinitrophenyl Group in Normal Human Sera. Nature 221, 960–962 (1969). https://doi.org/10.1038/221960a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/221960a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.