Abstract
CONCERNING the transcription of genetic information by DNA, recent investigations, using systems of the transformation of D. pneumoniae1 and infection with some bacteriophages2–4, have demonstrated that the DNA molecule consists of an informational and a non-informational strand. Guild and Robinson1 succeeded in obtaining fractions of denatured transforming DNA in CsCl density-gradient centrifugation which seemed to correspond to each polynucleotide chain, and they examined the time course of the appearance of cells possessing the new phenotype. It was observed that the expression of the new phenotype by one fraction was retarded for about one generation period compared with the other fraction. The messenger RNA synthesized after infection with bacteriophages2,3, or the RNA synthesized enzymatically in an in vivo system2,4, were examined for hybrid formation with polynucleotide chains separated in CsCl density-gradient centrifugation. The data suggested that the messenger RNA produced in vivo can form a ribonuclease-resistant hybrid with the unique polynucleotide chain of the primer DNA.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Guild, W. R., and Robinson, M., Proc. U.S. Nat. Acad. Sci., 50, 106 (1963).
Tocchini-Valentini, G. P., Stodolsky, S., Sarnat, A., Graziosi, F., Weiss, S. B., and Guiduchek, E. P., Proc. U.S. Nat. Acad. Sci., 50, 935 (1963).
Marmur, J., and Greenspan, C., Science, 142, 387 (1963).
Hayashi, M., Hayashi, M. N., and Spiegelman, S., Proc. U.S. Nat. Acad. Sci., 51, 351 (1964).
Hotchkiss, R. D., in Chemical Basis of Heredity, edit. by W. D. McElroy and B. Glass, 321 (Johns Hopkins Press, 1957).
Lacks, D., J. Mol. Biol., 5, 119 (1962).
Ephrussi-Taylor, H., Exp. Cell Res., 2, 589 (1951).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
SUZUKI, K., YAMAGAMI, H. & SHIMAZU, Y. Effect of Mitomycin C on Early Phenotypic Expression in the Transformation of Diplococcus pneumoniae. Nature 205, 929–930 (1965). https://doi.org/10.1038/205929a0
Issue Date:
DOI: https://doi.org/10.1038/205929a0
This article is cited by
-
Excision and repair of mismatched base pairs in transformation of Streptococcus pneumoniae
Molecular and General Genetics MGG (1980)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
