Abstract
THIN-LAYER chromatography on cellulose layers has been shown to be an effective method for separation and quantitative determination of nucleic acid bases, nucleosides and nucleotides1–5 which has considerable advantages over paper chromatography. Keck and Hagen5 recently reported a one-dimensional thin-layer chromatographic separation of the four major bases of RNA and DNA. The present communication describes a two-dimensional procedure which in this laboratory has proved useful for separating more complex mixtures of nucleic acid bases.
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RANDERATH, K. Two-dimensional Separation of Nucleic Acid Bases on Cellulose Layers. Nature 205, 908 (1965). https://doi.org/10.1038/205908a0
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DOI: https://doi.org/10.1038/205908a0
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