Abstract
THE phenol–hypochlorite reaction for ammonia was first described by D. D. Van Slyke and A. Hiller1, and later was modified by M. Borsook2 and J. A. Russell3. Unfortunately this reaction cannot be applied directly to biological fluids, because certain amino-acids, alkylamines and ammonium salts can react to a certain extent. Moreover, Russell's modification, being dependent on small concentrations of molybdenum ion, can be affected by the presence of thiols, thioamides, and in general by any chelating andreducing substances.
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References
Van Slyke, D. D., and Hiller, A., J. Biol. Chem., 102, 499 (1933).
Borsook, M., J. Biol. Chem., 110, 481 (1935).
Russell, J. A., J. Biol. Chem., 156, 457 (1944).
Philpot, J. S. L., and Small, P. A., Biochem. J., 32, 542 (1938).
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MUFTIC, M. A New Phenol–Hypochlorite Reaction for Ammonia. Nature 201, 622–623 (1964). https://doi.org/10.1038/201622a0
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DOI: https://doi.org/10.1038/201622a0
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