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Preparation of an Electron-dense Antibody Conjugate

Abstract

THE fluorescent antibody conjugate technique recently developed by Coons1 has already proved of great value in the study of biological problems. In this technique, a fluorescent substance is covalently linked to an antibody, and the antibody conjugate is allowed to react with cells or tissue containing its homologous antigen. The antigen is then detected and localized by observing the fluorescence in an optical microscope. For many reasons, it is desirable to extend this method to the sub-cellular level with the resolution attainable in the electron microscope. In order to achieve this purpose, however, it is necessary to confer sufficient electron density upon an antibody molecule, without inactivating it, to render it visible in the electron microscope.

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References

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SINGER, S. Preparation of an Electron-dense Antibody Conjugate. Nature 183, 1523–1524 (1959). https://doi.org/10.1038/1831523a0

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  • DOI: https://doi.org/10.1038/1831523a0

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