Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letters to Editor
  • Published:

Multiple Length DNA Molecules of Bacteriophage φX174

Abstract

RUSH and Warner have suggested that circular double length DNA molecules1–3 of bacteriophage S13 arise during genetic recombination, presumably in a single reciprocal recombination event4–6 between two monomeric circles7. In other systems, however, circular molecules of multiple length are produced by errors in replication8,9. We now demonstrate that most circular multiple length DNA molecules of bacteriophage φX174 arise by replication and that, moreover, some catenated DNA molecules contain interlocked rings of two different parental types.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

References

  1. Burton, A., and Sinsheimer, R. L., J. Mol. Biol., 14, 327 (1965).

    Article  CAS  Google Scholar 

  2. Rush, M. G., Kleinschmidt, A. T., Hellman, W., and Warner, R. C., Proc. US Nat. Acad. Sci., 58, 1676 (1967).

    Article  CAS  Google Scholar 

  3. Rush, M. G., and Warner, R. C., Proc. US Nat. Acad. Sci., 58, 2372 (1967).

    Article  CAS  Google Scholar 

  4. Gordon, C. N., Rush, M. G., and Warner, R. C., J. Mol. Biol., 47, 495 (1970).

    Article  CAS  Google Scholar 

  5. Rush, M. G., and Warner, R. C., J. Biol. Chem., 243, 4821 (1968).

    CAS  PubMed  Google Scholar 

  6. Rush, M. G., and Warner, R. C., Cold Spring Harbor Symp. Quant. Biol., 33, 459 (1968).

    Article  CAS  Google Scholar 

  7. Hudson, B., and Vinograd, J., Nature, 216, 647 (1967).

    Article  CAS  Google Scholar 

  8. Goebel, W., and Helinski, D. R., Proc. US Nat. Acad. Sci., 61, 1406 (1968).

    Article  CAS  Google Scholar 

  9. Helinski, D. R., and Clewell, D. B., Ann. Rev. Biochem., 40, 899 (1971).

    Article  CAS  Google Scholar 

  10. Davis, R. W., Simon, M., and Davidson, N., Methods in Enzymol., 21D, 413 (1971).

    Article  Google Scholar 

  11. Benbow, R. M., Hutchison, C. A., Fabricant, J. D., and Sinsheimer, R. L., J. Virol., 7, 549 (1971).

    CAS  PubMed  PubMed Central  Google Scholar 

  12. Lindqvist, B., and Sinsheimer, R. L., J. Mol. Biol., 28, 87 (1967).

    Article  CAS  Google Scholar 

  13. Levine, A. J., and Sinsheimer, R. L., J. Mol. Biol., 39, 619 (1969).

    Article  CAS  Google Scholar 

  14. Levine, A. J., and Sinsheimer, R. L., J. Mol. Biol., 32, 567 (1968).

    Article  CAS  Google Scholar 

  15. Calendar, R., Lindqvist, B., Sironi, Or., and Clark, A. J., Virology, 40, 72 (1970).

    Article  CAS  Google Scholar 

  16. Tessman, I., Biochem. Biophys. Res. Commun., 11, 169 (1966).

    Article  Google Scholar 

  17. Kim, J. S., Sharp, P., and Davidson, N., Proc. US Nat. Acad. Sci. (in the press).

  18. Boon, T., and Zinder, N. D., J. Mol. Biol., 58, 133 (1971).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

BENBOW, R., EISENBERG, M. & SINSHEIMER, R. Multiple Length DNA Molecules of Bacteriophage φX174. Nature New Biology 237, 141–144 (1972). https://doi.org/10.1038/newbio237141a0

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1038/newbio237141a0

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing