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Automated Cas13-based detection of SARS-CoV-2 in saliva
This issue highlights point-of-care assays and sequencing technology for the detection of infectious agents and for the study of host responses to infectious diseases.
The cover illustrates a microfluidic cartridge for the automated amplification and detection of SARS-CoV-2 RNA in saliva via the Cas13-mediated cleavage of a quenched fluorophore.
Assays leveraging the CRISPR-associated enzyme Cas13 and isothermal RNA amplification for the detection of viral RNA are being simplified for point-of-care use.
An assay leveraging strand-displacement reactions and enzymatic amplification for the recognition of viral RNA and implemented on origami paper allows for the fast colorimetric detection of SARS-CoV-2 variants, with single-nucleotide specificity.
Multiplex detection of two interacting Mycobacterium tuberculosis biomarkers on the surface of circulating extracellular vesicles, using a nanoplasmon-enhanced immunoassay, improves the diagnosis of tuberculosis in immunosuppressed children living with HIV.
SARS-CoV-2 and its variants can be visually detected via easy-to-use Cas13-based nucleic acid tests leveraging lyophilised reagents and fast sample inactivation at ambient temperature.
A COVID-19 test implemented in an automated microfluidic device and leveraging isothermal RNA amplification followed by T7 transcription and Cas13-mediated cleavage of a quenched fluorophore rapidly detects SARS-CoV-2 RNA in saliva samples.
A paper-based assay leveraging nucleic acid strand-displacement reactions and the enzymatic amplification of the recognition of viral RNA at the single-nucleotide level allows for the rapid colorimetric detection of SARS-CoV-2 variants.
A 3D-printed lab-on-a-chip allows for the concurrent rapid electrochemical detection of SARS-CoV-2 RNA in saliva and of anti-SARS-CoV-2 antibodies in saliva spiked with blood plasma.
The optical detection of two virulence factors of Mycobacterium tuberculosis on extracellular vesicles in blood allowed for the diagnosis of tuberculosis in paediatric cases that were missed by clinical microbiological assays.
Pathogenic autoreactive antibodies associated with severe COVID-19 can be identified via self-assembled genome-scale libraries of full-length proteins covalently coupled to uniquely identifying DNA barcodes for analysis by sequencing.
HIV proviruses and their adjacent host DNA junctions can be assembled via high-throughput droplet-based whole-genome amplification followed by a polymerase chain reaction to tag droplets containing proviruses for sequencing.