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Biomed-2

Human T-cell lines with well-defined T-cell receptor gene rearrangements as controls for the BIOMED-2 multiplex polymerase chain reaction tubes

Leukemia volume 21, pages 230–237 (2007)Cite this article

Abstract

The BIOMED-2 multiplex polymerase chain reaction (PCR) tubes for analysis of immunoglobulin and T-cell receptor (TCR) gene rearrangements have recently been introduced as a reliable and easy tool for clonality diagnostics in suspected lymphoproliferations. Quality and performance assessment of PCR-based clonality diagnostics is generally performed using human leukemia/lymphoma cell lines as controls. We evaluated the utility of 30 well-defined human T-cell lines for quality performance testing of the BIOMED-2 PCR primers and protocols. The PCR analyses of the TCR loci were backed up by Southern blot analysis. The clonal TCRB, TCRG and TCRD gene rearrangements were analyzed for gene segment usage and for the size and composition of their junctional regions. In 29 out of 30 cell lines, unique clonal TCR gene rearrangements could be easily detected. Besides their usefulness in molecular clonality diagnostics, these cell lines can now be authenticated based on their TCR gene rearrangement profile. This enables their correct use in molecular clonality diagnostics and in other cancer research studies.

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Acknowledgements

We gratefully acknowledge Marieke Comans-Bitter for performing the immunophenotypical analysis.

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Authors and Affiliations

  1. Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands

    Y Sandberg, B Verhaaf, E J van Gastel-Mol, I L M Wolvers-Tettero, J de Vos, J G Noordzij, W A Dik, J J M van Dongen & A W Langerak

  2. DSMZ – German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany

    R A F MacLeod

Authors
  1. Y Sandberg
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  2. B Verhaaf
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  3. E J van Gastel-Mol
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  4. I L M Wolvers-Tettero
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  5. J de Vos
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  6. R A F MacLeod
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  9. J J M van Dongen
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  10. A W Langerak
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Correspondence to J J M van Dongen.

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Funding sources: none.

Supplementary Information accompanies the paper on the Leukemia website (http://www.nature.com/leu)

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Sandberg, Y., Verhaaf, B., van Gastel-Mol, E. et al. Human T-cell lines with well-defined T-cell receptor gene rearrangements as controls for the BIOMED-2 multiplex polymerase chain reaction tubes. Leukemia 21, 230–237 (2007). https://doi.org/10.1038/sj.leu.2404486

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