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Human cord blood long-term engrafting cells are CD34+ CD38−

Leukemia volume 17, pages 960–964 (2003)Cite this article

Abstract

There have been controversies about CD34 and CD38 expression by human cord blood (CB) stem cells. Using the newborn NOD/SCID/β2-microglobulin-null mouse assay that we recently developed, we examined the in vivo engrafting capability of human CB cells. Almost all of the 4–5 months engrafting cells were found in CD34+ population. The capability of secondary reconstitution was found only in the CD34+ cells. When the CD34+ CB cells were separated into CD38− and CD38+ subpopulations and tested for engraftment, the majority of the engrafting cells were detected in the CD38− subpopulation. These findings are consistent with the results from studies of murine stem cells and strongly indicate that the phenotype of human CB stem cells is CD34+ CD38−.

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Acknowledgements

We thank Krista E Harris and Deidre R Wright for the excellent care of immunocompromised mice.

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Authors and Affiliations

  1. Department of Veterans Affairs Medical Center, Department of Medicine, Medical University of South Carolina, Charleston, SC, USA

    F Ishikawa, A G Livingston, H Minamiguchi & M Ogawa

  2. Division of Hematology/Oncology, University of Florida, Gainesville, FL, USA

    J R Wingard

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  1. F Ishikawa
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  2. A G Livingston
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  3. H Minamiguchi
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  4. J R Wingard
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  5. M Ogawa
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Additional information

This work was supported by the Office of Research and Development, Medical Research Services, Department of Veterans Affairs and by the Grants PO1-CA78582 and RO1-DK54197 from the National Institutes of Health

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Ishikawa, F., Livingston, A., Minamiguchi, H. et al. Human cord blood long-term engrafting cells are CD34+ CD38−. Leukemia 17, 960–964 (2003). https://doi.org/10.1038/sj.leu.2402878

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  • DOI: https://doi.org/10.1038/sj.leu.2402878

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