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SHP-1 expression in peripheral T cells from patients with Sezary syndrome and in the T cell line HUT-78: implications in JAK3-mediated signaling

Leukemia volume 16, pages 1470–1477 (2002)Cite this article

Abstract

SHP-1 is a key tyrosine phosphatase that acts as a negative regulator of signal transduction in lymphocytes, which has been found down-regulated in several T cell lines derived from human T cell malignancies. The standardization of a sensitive ELISA for the quantification of SHP-1 protein in peripheral T and B lymphocytes has enabled us to quantify the SHP-1 content of freshly isolated T cells from patients with Sezary syndrome and in the Sezary T cell line HUT-78. In all cases, a dramatic decrease in the content of this protein, when compared with the content in healthy volunteer controls, was observed. These results were corroborated when the expression of SHP-1 mRNA was analyzed. In order to study whether there was any correlation between SHP-1 protein expression and tyrosine phosphorylated state of JAK3, the state of phosphorylation of JAK3 was studied in the T cell line HUT-78, and found to be highly phosphorylated. These results suggest that SHP-1 might be involved in maintaining the IL-2R/JAK3 signaling pathway under control and point towards a role of SHP-1 in the pathogenesis of the disease.

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Acknowledgements

This work was supported by the FIS grants 00/0196 and 00/410.

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Authors and Affiliations

  1. Servicio de Immunología, Hospital Ramón y Cajal, Madrid, Spain

    F León, C Cespón, A Franco, M Lombardía, E Roldán, P González-Porqué & G Roy

  2. Servicio de Hematología, Hospital Ramón y Cajal, Madrid, Spain

    L Escribano

  3. Servicio de Dermatología, Hospital Ramón y Cajal, Madrid, Spain

    A Harto

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  1. F León
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León, F., Cespón, C., Franco, A. et al. SHP-1 expression in peripheral T cells from patients with Sezary syndrome and in the T cell line HUT-78: implications in JAK3-mediated signaling. Leukemia 16, 1470–1477 (2002). https://doi.org/10.1038/sj.leu.2402546

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