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Molecular Cytogenetics

Molecular analysis of the CALM/AF10 fusion: identical rearrangements in acute myeloid leukemia, acute lymphoblastic leukemia and malignant lymphoma patients

Leukemia volume 14, pages 93–99 (2000)Cite this article

Abstract

The recurring translocation t(10;11)(p13;q14) which is found in acute myeloid leukemia (AML) and in acute lymphoblastic leukemia (ALL) results in the fusion of the putative transcription factor AF10 to CALM encoding a clathrin assembly protein. Previous studies using mainly fluorescence in situ hybridization (FISH) analysis have shown that the CALM/AF10 rearrangement is found in immature acute myeloid leukemia (AML) of subtype M0 and M1 and in T cell ALL. In this study we analyzed the CALM/AF10 and AF10/CALM fusion mRNAs in a series of three patients with AML, one patient with T-ALL and two patients with precusor T lymphoblastic lymphoma. In all six patients the breakpoint in CALM is at the 3′ end of the coding region (nt1926/1927 or nt 2091/2092). Three breakpoints could be identified in AF10 (nt 588/589, nt 882/883 and nt 978/979). These data demonstrate that the CALM/AF10 fusions found in patients differ only slightly with respect to the portion of AF10 present and that there is no obvious difference between the fusions found in AML patients compared to those found in patients with lymphoid malignancies.

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Acknowledgements

We would like to thank Dr JD Rowley and Dr K Carlson for critically reading this manuscript and sharing prepublication data with us.

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Authors and Affiliations

  1. Institute of Human Genetics, Georg-August University, Göttingen, Germany

    SK Bohlander & V Muschinsky

  2. Department of Internal Medicine, Georg-August University, Göttingen, Germany

    K Schrader

  3. Institute of Human Genetics, University of Kiel, Kiel, Germany

    R Siebert, B Schlegelberger & L Harder

  4. Department of Pediatrics, NHL-BFM-Studienzentrale, Medizinische Hochschule Hannover, Hannover, Germany

    V Schemmel

  5. Institute of Medical Biology, University of Vienna, Vienna, Austria

    C Fonatsch

  6. Department of Hematology/Oncology and Tumor Immunology, Robert-Rössle-Hospital, Humboldt University, Berlin, Germany

    W-D Ludwig

  7. Department of Internal Medicine, Ludwigs-Maximilian University, Munich, Germany

    W Hiddemann & MH Dreyling

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Bohlander, S., Muschinsky, V., Schrader, K. et al. Molecular analysis of the CALM/AF10 fusion: identical rearrangements in acute myeloid leukemia, acute lymphoblastic leukemia and malignant lymphoma patients. Leukemia 14, 93–99 (2000). https://doi.org/10.1038/sj.leu.2401614

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  • DOI: https://doi.org/10.1038/sj.leu.2401614

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