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New Cell Line

A new human cell line with pre-B cell phenotype and t(5;12)

Leukemia volume 11, pages 1040–1044 (1997)Cite this article

Abstract

A new cell line (LR10.6) with pre-B cell phenotype has been established from bone marrow cells obtained from a child with B lineage acute lymphoblastic leukemia in complete clinical remission. The line expresses nuclear TdT enzyme, cytoplasmic Igλ-chain and membrane μ-chain and other B but no T or myeloid markers. The cells also show activation antigens CD69 and CD71, adhesion molecules CD54, CD50 and CD56 and the tyrosine kinase receptor CD117. No expression of multidrug resistance phenotype MDR-1 is observed on these cells which nevertheless express the transcriptional factor p53 protein in a mutant form. Cytogenetic study shows a translocation t(5;12)(q31;p13) involving breakpoints which contain the growth factor interleukin 3 gene (5q31) and the recently identified TEL/ETV6 gene (12p13). Activation of the cells with phorbol-12 myristate 13-acetate (PMA) up-regulates the expression of the CD69 activation antigen and down-regulates the CD117 molecule. In addition, PMA fails to induce the CD20 B cell antigen.

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Authors and Affiliations

  1. Cryobiology and Cell Therapy Department of Institut de Recerca Oncologica (IRO), Spain

    J Inglés-Esteve, C Azqueta, E Kádár, KM Bothner, B Amill, A Limón & J García López

  2. Department of Hematology, Hospital Sant Pau, Barcelona, Spain

    A Aventín

Authors
  1. J Inglés-Esteve
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  2. A Aventín
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  3. C Azqueta
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  4. E Kádár
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  5. KM Bothner
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  6. B Amill
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  7. A Limón
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  8. J García López
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Inglés-Esteve, J., Aventín, A., Azqueta, C. et al. A new human cell line with pre-B cell phenotype and t(5;12). Leukemia 11, 1040–1044 (1997). https://doi.org/10.1038/sj.leu.2400692

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  • DOI: https://doi.org/10.1038/sj.leu.2400692

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