Volume 49 Issue 11, November 2020

Host genetics, housing conditions, and antibiotic treatments impact microbiota diversity and composition, but microbes that inhabit the mouse gut and skin can also influence severity and penetrance of host mutant phenotypes.

Journeying into the field to study a model organism in its natural habitats.

To achieve knockdown rather than knockout of particular genes, a new paper demonstrates a CRISPR/Cas13 method that can efficiently edit mRNA in zebrafish, medaka, killifish, and mouse embryos.

This protocol describes the different steps to establish a murine model of restenosis after angioplasty of a stenotic arteriovenous fistula (AVF), including a detailed description of the partial nephrectomy procedure to induce chronic kidney disease, the AVF procedure for development of de novo stenosis and the angioplasty treatment associated with progression of restenosis.