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Upregulation of Neutrophil Surface Adhesion Molecules in Infants of Pre-eclamptic Women

Journal of Perinatology volume 24, pages 208–212 (2004)Cite this article

Abstract

OBJECTIVE: To evaluate evidence of neutrophil activation in infants born to pre-eclamptic women and examine any association between degree of neutrophil activation and severity of pre-eclampsia.

DESIGN: This study utilized quantitative flow cytometry to determine whether the expression of surface adhesion molecules: CD18, CD11a, CD11b, and CD11c on cord blood neutrophils using mean channel fluorescence values (MCF). A total of 20 infants of pre-eclamptic women were compared with a control group of 19 infants of normotensive women.

RESULTS: MCF values were significantly higher in infants born to pre-eclamptic women vs controls: CD18 (432.0±236.3 vs 230.5±97.9; p=0.002), CD11a (552.9±272.4 vs 326.9±268.6; p=0.003), CD11b (937.2±521.9 vs 576.6±352.9; p=0.025), and CD11c (228.5±130.3 vs 133.0±77.1; p=0.006), respectively. The mean MCF values appeared higher in severe vs mild pre-eclampsia.

CONCLUSIONS: This study revealed neutrophil activation in infants born to pre-eclamptic women. The relationship between neutrophil activation and severity of pre-eclampsia warrants further study.

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Acknowledgements

This study was supported by a grant from the Park Ridge Health Foundation. Special thanks go to those who assisted with this project: Colleen Loewe (ACL Laboratories), Bryan Coyle, MA (Analyst, Advocate Health Care IRB), and Lorene Schweig, RN (NICU Research Coordinator).

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Authors and Affiliations

  1. Division of Neonatology, Department of Pediatrics, Lutheran General Children's Hospital, Park Ridge, IL, USA

    Hemant Saini MD & Bhagya L Puppala MD

  2. Advocate Medical Group, Park Ridge, IL, USA

    Bhagya L Puppala MD

  3. Division of Research, Department of Pediatrics, Advocate-Lutheran General Children's Hospital, Park Ridge, IL, USA

    Denise Angst DNSc

  4. FUHS/Chicago Medical School Department of Microbiology and Immunology, Chicago, IL, USA

    Alice Gilman-Sachs PhD

  5. Department of Immunovirology, ACL Laboratories, Park Ridge, IL, USA

    Michael Costello PhD

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  1. Hemant Saini MD
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  2. Bhagya L Puppala MD
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  3. Denise Angst DNSc
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  4. Alice Gilman-Sachs PhD
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Financial support: Park Ridge Health Foundation.

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Saini, H., Puppala, B., Angst, D. et al. Upregulation of Neutrophil Surface Adhesion Molecules in Infants of Pre-eclamptic Women. J Perinatol 24, 208–212 (2004). https://doi.org/10.1038/sj.jp.7211056

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