Pediatric Highlight
International Journal of Obesity (2009) 33, 758–767; doi:10.1038/ijo.2008.260; published online 9 December 2008
Shifts in clostridia, bacteroides and immunoglobulin-coating fecal bacteria associated with weight loss in obese adolescents
I Nadal1, A Santacruz1, A Marcos2, J Warnberg2, M Garagorri3, L A Moreno4, M Martin-Matillas5, C Campoy5, A Martí6, A Moleres6, M Delgado7, O L Veiga8, M García-Fuentes9, C G Redondo9 and Y Sanz1
- 1Microbial Ecophysiology, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Valencia, Spain
- 2Grupo de Inmunonutrición, Instituto del Frío. ICTAN (CSIC), Madrid, Spain
- 3Departamento de Pediatría, Radiología y Medicina Física, Universidad de Zaragoza, Spain
- 4E.U. Ciencias de la Salud, Universidad de Zaragoza, Spain
- 5Facultad de Medicina, Universidad de Granada, Granada, Spain
- 6Departamento de Fisiología y Nutrición, Universidad de Navarra, Pamplona Spain
- 7Facultad de Ciencias de la Actividad Física y Deporte, Universidad de Granada, Granada, Spain
- 8Departamento de Educación Física, Deporte y Movimiento Humano, Universidad Autónoma de Madrid, Spain
- 9Departamento de Ciencias Médicas y Quirúrgicas, Universidad de Cantabria, Santander, Spain
Correspondence: Dr Y Sanz, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Apartado 73, Burjassot, Valencia 46100, Spain. E-mail: yolsanz@iata.csic.es
Received 10 April 2008; Revised 26 October 2008; Accepted 2 November 2008; Published online 9 December 2008.
Abstract
Objective:
To evaluate the effects of a multidisciplinary obesity treatment programme on fecal microbiota composition and immunoglobulin-coating bacteria in overweight and obese adolescents and their relationship to weight loss.
Design:
Longitudinal intervention study based on both a calorie-restricted diet (calorie reduction=10–40%) and increased physical activity (calorie expenditure=15–23 kcal/kg body weight per week) for 10 weeks.
Participants:
Thirty-nine overweight and obese adolescents (BMI mean 33.1 range 23.7–50.4; age mean 14.8 range, 13.0–16.0).
Measurements:
BMI, BMI z-scores and plasma biochemical parameters were measured before and after the intervention. Fecal microbiota was analyzed by fluorescent in situ hybridization. Immunoglobulin-coating bacteria were detected using fluorescent-labelled F(ab')2 antihuman IgA, IgG and IgM.
Results:
Reductions in Clostridium histolyticum and E. rectale-C. coccoides proportions significantly correlated with weight and BMI z-score reductions in the whole adolescent population. Proportions of C. histolyticum, C. lituseburense and E. rectale-C. coccoides dropped significantly whereas those of the Bacteroides-Prevotella group increased after the intervention in those adolescents who lost more than 4 kg. Total fecal energy was almost significantly reduced in the same group of adolescents but not in the group that lost less than 2.5 kg. IgA-coating bacterial proportions also decreased significantly in participants who lost more than 6 kg after the intervention, paralleled to reductions in C. histolyticum and E. rectale-C. coccoides populations. E. rectale-C. coccoides proportions also correlated with weight loss and BMI z-score reduction in participants whose weight loss exceeded 4 kg.
Conclusions:
Specific gut bacteria and an associated IgA response were related to body weight changes in adolescents under lifestyle intervention. These results suggest interactions between diet, gut microbiota and host metabolism and immunity in obesity.
Keywords:
overweight, microbiota, weight management, IgA
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