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The G1057D polymorphism of IRS-2 gene and its relationship with obesity in conferring susceptibility to type 2 diabetes in Asian Indians

International Journal of Obesity volume 31, pages 97–102 (2007)Cite this article

Abstract

Objective:

To investigate the association of insulin receptor substrate-2 (IRS-2) G1057D polymorphism with type 2 diabetes and obesity in Asian Indians.

Methods:

The study comprised of 1193 normal glucose tolerant (NGT) subjects and 1018 subjects with type 2 diabetes, aged ⩾20 years with an average body mass index of 23.7±4.6 and 25.3±4.2 kg/m2, respectively. The subjects were unrelated and randomly selected from the Chennai Urban Rural Epidemiology Study (CURES), a population-based study in Chennai in southern India. The G1057D polymorphism of the IRS-2 gene was genotyped using PCR-RFLP assay.

Results:

The genotype frequency of the IRS-2 G1057D polymorphism was significantly different between the NGT and type 2 diabetic groups (P=0.0007) in the total study subjects and among the obese subjects (P=0.00007). Logistic regression analysis showed that the DD genotype showed an increased susceptibility to diabetes with an odds ratio (adjusted for age and sex) of 2.19 (95% CI: 1.34–3.57, P=0.002) when compared to the GG+GD genotype, among the obese subjects, but not in non obese subjects. In order to explore possible interaction with obesity, logistic regression analysis was performed and the coefficient corresponding to the interaction parameter (genotype × obesity) was significant (P=0.0001).

Conclusion:

In Asian Indians, the DD genotype increases susceptibility to type 2 diabetes by interacting with obesity.

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Abbreviations

IRS-2:

insulin receptor substrate-2

CURES:

Chennai Urban Rural Epidemiology Study

NGT:

normal glucose tolerance

OR:

odds ratio.

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Acknowledgements

The financial support offered by the Department of Biotechnology (DBT), Government of India and the help of Dr Reddy's Laboratories Limited, Hyderabad, India, to set up the genetics lab at our centre are gratefully acknowledged. We also thank the Chennai Wellingdon Corporate Foundation, Chennai, for their financial support for the CURES field study. This is the 19th publication from the CURES study (CURES 19).

Author information

Authors and Affiliations

  1. Madras Diabetes Research Foundation and Dr Mohan's Diabetes Specialities Centre, Gopalapuram, Chennai, India

    D Bodhini, V Radha, R Deepa & V Mohan

  2. Human Genetics Unit, Indian Statistical Institute, Kolkata, India

    S Ghosh & P P Majumder

  3. Department of Biochemistry, Indian Institute of Science, Bangalore, India

    M R S Rao

  4. Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India

    M R S Rao

Authors
  1. D Bodhini
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  2. V Radha
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  3. R Deepa
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  4. S Ghosh
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  5. P P Majumder
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  6. M R S Rao
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  7. V Mohan
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Correspondence to V Radha.

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Bodhini, D., Radha, V., Deepa, R. et al. The G1057D polymorphism of IRS-2 gene and its relationship with obesity in conferring susceptibility to type 2 diabetes in Asian Indians. Int J Obes 31, 97–102 (2007). https://doi.org/10.1038/sj.ijo.0803356

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