Abstract
A variety of karyotypic differences were seen among six Lathyrus species. In part these differences are the result of different numbers of metacentric and acrocentric chromosomes in the complement but there were also clear differences in the number(s) and position(s) of secondary constrictions. Silver staining and in-situ hybridization were used to identify the nucleolar organizer regions (NORs). Silver staining also occurred at the centromere in most species and in addition at a number of non-centromeric sites in L. tingitanus. Some of these additional sites coincide with allocyclic regions revealed by the use of air-dried preparations. Unusual ‘extended’ centromeres were observed in these air-dried preparations of two of the species.
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Bhattacharjee, S K. 1954. Cytogenetics of Lathyrus sativus Linn. Caryologia, 6, 333–337.
Darlington, C D. 1926. Chromosome studies in the Scilleae. J Genet, 16, 237–251.
Fouzdar, A, and Tandon, S L. 1975. Cytotaxonomic investigations in the genus Lathyrus. Nucleus, 18, 24–44.
Geber, G, and Schweizer, D. 1988. Cytochemical heterochromatin differentiation in Sinapis alba using a simple air-drying technique for producing chromosome spreads. Plant Syst Evol, 158, 97–106.
Gerlach, W L, and Bedbrook, J R. 1979. Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res, 7, 1869–1885.
Haaf, T, Weis, H, Schindler, D, and Schmid, M. 1984. Specific silver staining of experimentally undercondensed chromosome regions. Chromosoma, 90, 149–155.
Heywood, V H. 1978. Flowering Plants of the World. Oxford, Oxford University Press.
Jackson, R C. 1971. The karyotype in systematics. Ann Rev Ecol & Syst, 2, 327–368.
Jimenez, R, Burgos, M, and Diaz De La Guardia, R. 1988. A study of the Ag-staining significance of mitotic NOR's. Heredity, 60, 125–127.
King, M, Contreras, N, and Honeycutt, R L. 1990. Variation within and between nucleolar organizer regions in Australian hylid frogs (Anura) shown by 18S + 28S in situ hybridization. Genetica, 80, 17–29.
Lavania, U C, and Sharma, A K. 1980. Giemsa C banding in Lathyrus. Bot Gaz, 141, 199–203.
Mukai, Y, Endo, T R, and Gill, B S. 1991. Physical mapping of the 18S.26S rRNA multigene family in common wheat: Identification of a new locus. Chromosoma, 100, 71–78.
Murray, B G, and Hammett, K R W. 1989. Lathyrus chloranthus × L. chrysanthus: A new interspecific hybrid. Bot Gaz, 150, 469–476.
Murray, B G, Hammett, K R W, and Standring, L S. 1992. Genomic constancy during the development of Lathyrus odoratus cultivars. Heredity, 68, 321–327.
Narayan, R K J, and Durrant, A. 1983. DNA distribution in chromosomes of Lathyrus species. Genetica, 61, 47–53.
Nazeer, M A, Subrahmanyam, G V, and Ohri, D. 1982. Intraspecific variation in chromsome phenotype and meiotic system in Lathyrus odoratus L. Cytologia, 47, 287–293.
Roy, R P, and Singh, M K. 1967. Cytological studies in the genus Lathyrus. J Cytol Genet, 2, 128–140.
Schubert, I, and Wobus, U. 1985. In situ hybridization confirms jumping nucleolus organizing regions in Allium. Chromosoma, 92, 143–148.
Sharma, A K, and Datta, P C. 1959. Application of improved technique in tracing karyotype differences between strains of Lathyrus odoratus L. Cytologia, 24, 389–402.
Stebbins, G L. 1950. Variation and evolution in plants. London, Oxford University Press.
Sudman, P D, and Greenbaum, I F. 1989. Visualization of kinetochores in mammalian meitotic preparations and observations of argentophilic differences between mitotic and meiotic kinetochores. Genome, 32, 380–382.
Verma, S C, and Ohri, D. 1979. Chromosome and nuclear phenotype in the legume Lathyrus sativus L. Cytologia, 44, 77–90.
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Murray, B., Bennett, M. & Hammett, K. Secondary constrictions and NORs of of Lathyrus investigated by silver staining and in-situ hybridization. Heredity 68, 473–478 (1992). https://doi.org/10.1038/hdy.1992.68
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DOI: https://doi.org/10.1038/hdy.1992.68
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