Abstract
Oryzias latipes and Oryzias curvinotus are closely related medaka species that have the common sex-determining gene, DMY, on their homologous Y chromosomes. We previously reported that sex-reversed XY females were produced in hybrids between O. curvinotus females and O. latipes males (Hd-rR inbred strain). In this study we used HNI inbred strain males of O. latipes for mating with O. curvinotus females, and found that all the XY hybrids developed as males. To map the factor responsible for this strain-specific XY sex reversal, O. curvinotus females were mated with two Y-congenic strains (HNI.YHd-rR and Hd-rR.YHNI) and a recombinant congenic strain (Hd-rR.YHNIrr). HNI.YHd-rR produced sex-reversed females in the XY hybrids, whereas no sex-reversed females were obtained in the XY hybrids from Hd-rR.YHNI and Hd-rR.YHNIrr, demonstrating that a small region on the Y chromosome, which includes DMY, is responsible for the XY sex reversal. Sex-reversed hybrids were only produced in the presence of the Y-chromosomal region derived from the Hd-rR strain, suggesting that missense or regulatory mutations specific to the Hd-rR Y-chromosomal region induce the sex reversal.
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Introduction
Mutations or new combinations of genes that give rise to phenotypic differences provide novel insights into genes and their functions in the molecular pathways underlying development. Consequently, inconsistencies between the genetic sex (XX or XY) and the phenotypic sex (male or female) are useful for identifying the genes involved in the sex-determination pathway. In mammals, the sex-determining gene, SRY/Sry, located on the Y chromosome, induces the undifferentiated gonad to develop as a testis (Gubbay et al., 1990; Sinclair et al., 1990; Koopman et al., 1991; Capel et al., 1993). Some human XY sex reversals with gonadal dysgenesis have deletions or mutations in coding or regulatory regions of the SRY gene, providing compelling evidence of its sex-determining function (Berta et al., 1990; Jager et al., 1990; Page et al., 1990). An essential role of Sox9 for testis differentiation was detected by analysis of XY sex-reversed patients with campomelic dysplasia (Foster et al., 1994). Furthermore, loss-of-function mutations in R-spondin1 cause XX male sex reversal in humans (Parma et al., 2006). Many other autosomal and X-chromosomal genes have been identified as having roles in the sex-differentiation pathway based on the analyses of human sex-reversal cases and functional analyses in mice (reviewed in Koopman, 2001).
In the medaka, Oryzias latipes, which has an XX/XY sex-determining system (Aida, 1921), DMY was found to be the Y-specific sex-determining gene (Matsuda et al., 2002, 2007). In this fish species, many sex-reversal mutants (XX males and XY females) have been found in wild populations (Shinomiya et al., 2004; Otake et al., 2006, 2008). All the observed XY sex-reversed females had Y-linked gene mutations and were classified into two groups: a group with mutations in the amino acid coding sequence of DMY and a group with an intact coding region but reduced DMY expression during critical periods for sex determination (Matsuda et al., 2002; Otake et al., 2006). These findings show that DMY is required for male sex determination. In addition, a genetic analysis of an XX male suggested the presence of autosomal modifiers for sexual differentiation of the gonads (Shinomiya et al., 2004).
Oryzias curvinotus is a closely related species to O. latipes, and also has an XX/XY sex-determining system (Hamaguchi et al., 2004). The DMY gene was identified on the homologous Y chromosome, and is only expressed in XY embryos (Matsuda et al., 2003). The process of gonadal sex differentiation in O. curvinotus is similar to that in O. latipes (Shinomiya et al., 2006), suggesting that O. curvinotus and O. latipes have a common sex-determining pathway. Furthermore, interspecific hybrids between O. curvinotus and O. latipes are viable and develop normal secondary sexual characteristics, although males are sterile and females lay diploid eggs (Hamaguchi and Sakaizumi, 1992; Sakaizumi et al., 1992, 1993). In a previous study, we analyzed the genetic and phenotypic sexes of hybrids between O. curvinotus females and O. latipes males of the Hd-rR inbred strain, and found that this mating yielded not only XX females and XY males, but also XY females (Shinomiya et al., 2006). Male fish only appeared in the XY hybrids, indicating that DMY is required for male determination of the hybrids. However, 21% of the XY hybrids were females, demonstrating that DMY cannot always determine maleness in the hybrids.
In this study, we used O. latipes males of the HNI inbred strain for mating with O. curvinotus females. Unlike the results with the Hd-rR strain, all the XY hybrids developed as males, indicating that the Hd-rR and HNI inbred strains differ in their ability for male determination in the XY hybrids. We also found that the Y chromosome of O. latipes was involved in the sex reversal in the XY hybrids by using two Y-congenic strains, HNI.YHd-rR and Hd-rR.YHNI, and a recombinant congenic strain, Hd-rR.YHNIrr, derived from Hd-rR.YHNI. Sex-reversed hybrids were only produced in the presence of the Y-chromosomal region derived from the Hd-rR strain, irrespective of the autosomal background, suggesting that divergence in the Y-chromosomal region is associated with the different sex-reversal ratios in the XY hybrids.
Materials and methods
Fish
We used a laboratory stock of O. curvinotus, as well as two inbred strains (HNI and Hd-rR), two Y-congenic strains (HNI.YHd-rR and Hd-rR.YHNI) and a recombinant Y-congenic strain (Hd-rR.YHNIrr) of O. latipes (Figure 1). All the strains were supplied by a sub-center (Niigata University, Niigata, Japan) of the National Bioresource Project (Medaka) (http://www.shigen.nig.ac.jp/medaka/). The wild stock of O. curvinotus was originally collected in Hong Kong in 1986 (Takehana et al., 2005). HNI and Hd-rR were established from Northern and Southern Japanese Populations, respectively (Hyodo-Taguchi and Sakaizumi, 1993). HNI.YHd-rR is a Y-congenic strain established from HNI females and an Hd-rR male. An F1 hybrid male was backcrossed to HNI females for 12 generations. This strain has the Y chromosome derived from Hd-rR on the HNI background. Similarly, Hd-rR.YHNI has the Y chromosome derived from HNI on the Hd-rR background (Matsuda et al., 1998). Hd-rR.YHNIrr is a recombinant strain derived from Hd-rR.YHNI, and has a small region of the Y chromosome of the HNI strain. This region is in an interval of 1.8 cM between two DNA markers, SL1 and 51H7.F, and includes DMY (Matsuda et al., 2002). The fish were maintained in aquaria under an artificial 14-h light:10-h dark photoperiod at 27±2 °C.
Mating and sexing
O. curvinotus females and O. latipes males of each strain were crossed by pair mating, and the naturally spawned eggs were collected and incubated under the same conditions as the adult fish. The hatched hybrid fish were reared for 2–3 months, and examined to determine their phenotypic and genotypic sexes.
Phenotypic sex was judged on the basis of secondary sex characteristics, namely, the shapes of the dorsal and anal fins. Genotypic sex (XY or XX) was determined on the basis of the presence or absence of the DMY gene, evaluated using PCR amplification of caudal fin clip genomic DNA extracted from adult fish. PCR amplification of DMY and DMRT1 was performed with the primers PG17.5 s (5′-CCGGGTGCCCAAGTGCTCCCGCTG-3′) and PG17.6U (5′-GATCGTCCCTCCACAGAGAAGAGA-3′) (Shinomiya et al., 2004) at an annealing temperature of 55 °C. The PCR products were analyzed by electrophoresis in a 1% agarose gel.
RNA extraction and reverse transcriptase PCR (RT-PCR)
Total RNA was extracted from fry at hatching using an RNeasy Mini Kit (Qiagen, Tokyo, Japan), and subjected to RT-PCR using a OneStep RT-PCR Kit (Qiagen). Aliquots (20 ng) of the total RNA samples were used as templates in 25-μl reaction volumes. The PCR amplification conditions were: 30 min at 55 °C; 15 min at 95 °C; 30 cycles of 30 s at 94 °C, 30 s at 55 °C and 30 s at 72 °C; and 10 min at 72 °C. For DMY, the initial PCR products were diluted by 1:200 and re-amplified. The conditions for the second PCR amplification were: 5 min at 95 °C; 22 cycles of 20 s at 94 °C, 30 s at 65 °C and 30 s at 72 °C; and 3 min at 72 °C. The specific primers used were as follows: DMY first amplification: PG17.5 s, 5′-CCGGGTGCCCAAGTGCTCCCGCTG-3′ and PG17.12U, 5′-GACCATCTCATTTTTTATTCTTGATTTTT-3′; DMY second amplification: DMYspe, 5′-TGCCGGAACCACAGCTTGAAGACC-3′ and 48U, 5′-GGCTGGTAGAAGTTGTAGTAGGAGGTTT-3′; β-actin: actin3b, 5′-CMGTCAGGATCTTCATSAGG-3′ and actin4, 5′-CACACCTTCTACAATGAGCTGA-3′ (Otake et al., 2006).
Results
Strain differences in hybrid XY sex reversal
In a previous study, we showed that sex-reversed XY females were produced from interspecific hybridization between O. curvinotus females and Hd-rR strain males of O. latipes (Shinomiya et al., 2006), as shown in Table 1. In brief, all 138 XX hybrids were females, whereas the 140 XY hybrids consisted of 110 males and 30 females, indicating that 21% of XY individuals developed as females in the hybrids.
In this study, we used O. latipes males of another inbred strain, HNI, for mating with O. curvinotus females, and analyzed the phenotypic and genotypic sexes of the F1 hybrids. The results showed that all 81 XX hybrids developed as females and all 70 XY hybrids developed as males, with no sex reversal (Table 1). The gonads of 68 XX females and 60 XY males were dissected out and observed using a stereomicroscope. All females had ovaries and all males had testes, indicating that HNI males did not produce sex-reversed XY females in the hybrids. These results suggest that the two inbred strains, HNI and Hd-rR, differ in their incidences of XY sex reversal in the interspecific hybrids.
Genetic mapping using Y-congenic strains
To test the possibility that the Y chromosome of O. latipes contributes to the strain-specific difference in the hybrid sex reversal, we mated males of two Y-congenic strains (HNI.YHd-rR and Hd-rR.YHNI) of O. latipes with O. curvinotus females (Table 1). HNI.YHd-rR males produced 42 XX hybrids that developed as females and 62 XY hybrids that consisted of 51 males (82%) and 11 females (18%). These findings indicated that HNI.YHd-rR males also produced sex-reversed XY females in the hybrids. In contrast, all 82 XX hybrids were females and all 75 XY hybrids were males in the mating with Hd-rR.YHNI males, indicating that Hd-rR.YHNI males did not produce sex-reversed XY females. These results clearly show that strain-specific differences in the Y chromosome of O. latipes contribute to the sex-reversal ratio in the XY hybrids.
To locate precisely the responsible region on the Y chromosome, we used Hd-rR.YHNIrr, a recombinant strain of Hd-rR.YHNI. This strain has a small region (including the sex-determining gene DMY) derived from the HNI strain on the Hd-rR genetic background (Matsuda et al., 2002). In this mating, all 110 XX hybrids developed as females and all 98 XY hybrids developed as males, demonstrating that the small region on the Y chromosome has a crucial role in the hybrid XY sex reversal.
Expression level of DMY
Reduced DMY expression levels have been observed in some XY sex-reversal mutants of O. latipes (Matsuda et al., 2002; Otake et al., 2006). To examine DMY expression in the hybrids during the sex-determining period, we performed RT-PCR analyses of fry at the hatching day. We analyzed the hybrids obtained from crosses between O. curvinotus and Hd-rR that produced sex reversal, and crosses between O. curvinotus and Hd-rR.YHNIrr and between O. curvinotus and HNI that produced no sex reversal. In addition, we analyzed the parental Hd-rR, Hd-rR.YHNIrr and HNI strains of O. latipes. Although DMY transcripts were detected in all XY fry, the expression levels were clearly lower in the XY hybrids between O. curvinotus and Hd-rR, compared with the other XY hybrids and the XY individuals of each strain (Figure 2). Similar reductions in the expression levels of DMY were also observed in the XY hybrids from crosses between O. curvinotus and Hd-rR at 10 and 15 days after hatching (data not shown).
Discussion
Our previous study showed that sex-reversed XY females were produced in the mating between O. curvinotus females and Hd-rR strain males of O. latipes (Shinomiya et al., 2006). However, this study has shown that no XY females were obtained in the hybrids between O. curvinotus females and HNI males. These findings indicate a strain difference in the ability to induce maleness in the XY hybrids. The two inbred strains, HNI and Hd-rR, were established from two regionally differentiated groups, namely Northern and Southern Japanese Populations, respectively (Sakaizumi et al., 1983; Takehana et al., 2003). They show high single-nucleotide polymorphism rates (Kasahara et al., 2007) and differ in terms of various quantitative traits, such as body shape, behavior and susceptibility to chemicals (Ishikawa, 2000). Furthermore, a recent analysis detected many differences in the craniofacial traits between the two inbred strains, and successfully identified chromosomal regions responsible for these traits using quantitative trait locus mapping (Kimura et al., 2007). Therefore, we consider that the hybrid XY sex reversal observed in this study is attributable to genetic differences between the HNI and Hd-rR strains, and that these differences make it possible to identify chromosomal regions associated with the sex reversal.
Using the two reciprocal Y-congenic strains (HNI.YHd-rR and Hd-rR.YHNI), we have clearly shown that the Y chromosome of O. latipes is responsible for the strain-specific difference in the hybrid sex reversal, as the former yielded XY sex reversal in the hybrids whereas the latter did not. Furthermore, a recombinant congenic strain (Hd-rR.YHNIrr) derived from the Hd-rR.YHNI strain produced no sex reversal in the hybrids, indicating that a small region of the Y chromosome contributes to the sex reversal. This critical region of the Y chromosome is in an interval of 1.8 cM between SL1 and 51H7.F, and contains the sex-determining gene, DMY (Matsuda et al., 2002). Taken together, these results suggest that the XY sex reversal in the hybrids results from incompatibility between the Hd-rR allele of the Y chromosomal locus and the O. curvinotus alleles of autosomal and/or X-chromosomal loci.
In O. latipes, mutations of DMY are associated with male-to-female sex reversal, and many mutations have been identified within the DMY open reading frame (Matsuda et al., 2002; Otake et al., 2006, 2008). Most of them were insertion/deletion mutations in the third exon of DMY, resulting in truncation of the DMY protein. As a consequence, all offspring that inherited these mutant alleles of DMY developed as XY females. On the other hand, some XY sex-reversal mutants with reduced expression of DMY produced both XY males and XY females in their progeny (Otake et al., 2006). The entire coding region of DMY was intact in these mutants, indicating that the XY sex reversal is associated with mutations that control DMY expression. Furthermore, the incidence of XY sex reversal was correlated with the DMY expression level, suggesting that a certain threshold level of DMY expression is required for male determination (Otake et al., 2006). These results suggest that the reduced expression of the DMY transcript can induce sex reversal in a subset of XY individuals having the same chromosome composition.
DMY expression first appears just before hatching, and morphological sex differentiation can be detected at hatching in both O. latipes and O. curvinotus (Matsuda et al., 2003; Kobayashi et al., 2004; Shinomiya et al., 2006). Our RT-PCR analyses indicated that the expression levels of DMY at hatching in the XYHd-rR hybrids were severely reduced compared with those in the XYHNI hybrids and in the XY individuals of the HNI and Hd-rR strains. This reduced expression suggests that the XY sex reversal in the hybrids could result from incompatibility between the cis-regulatory region of the DMYHd-rR allele and autosomal or X-linked trans-acting loci of O. curvinotus. However, further expression analyses of DMY by quantitative PCR or in situ hybridization are necessary to determine whether the DMY expression levels are responsible for the XY sex reversal in the interspecific hybrids.
Similar conditions were found in mice, as XY sex reversal occurred when a certain variant of the Mus musculus domesticus type Y chromosome (YDOM) was transferred onto the C57BL/6J (abbreviated to B6) inbred strain background (Eicher et al., 1982). This B6-YDOM sex reversal was classified into three groups based on the gonadal phenotypes (Bullejos and Koopman, 2005). The first group, represented by YPOS and YTIR, showed XY sex reversal with some ovaries or ovotestes on a B6 genetic background. The second group, represented by YAKR and YRF/J, had normal testes on a B6 genetic background, although the testis cord formation was delayed. The third group, represented by YFVB and YSJL, showed normal testis development on a B6 genetic background. It has been suggested that the B6-YDOM sex reversal is caused by abnormal interactions of B6-derived autosomal or X-linked loci with the M. musculus domesticus type Y chromosome. Furthermore, expression analyses showed reduced expression levels and delayed expression of Sry, which were correlated with the degree of sex reversal in each B6-YDOM strain, suggesting that regulatory mutations affecting the timing and/or levels of Sry expression are responsible for the sex reversal in B6-YDOM (Nagamine et al., 1999; Bullejos and Koopman, 2005).
As O. latipes and O. curvinotus are closely related species that are considered to have a common sex-determining pathway, sex reversal in their hybrids should arise from abnormal combinations of genes, rather than defective genes, in this pathway. Our results clearly showed that a Y-chromosomal region including DMY causes a strain-specific difference in the ability to induce maleness in the XY hybrids, suggesting that the hybrid sex reversal could result from incompatibility between this Y-chromosomal region of O. latipes and the O. curvinotus alleles of autosomal and/or X-chromosomal loci. Future analyses, including evaluation of the functional differences of DMY proteins among strains, analysis of the DMY expression patterns in the XY hybrids, and identification of the DMY regulatory elements and the factors controlling DMY expression, will help to elucidate the molecular mechanism of the DMY action and sex reversal in the hybrids.
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Acknowledgements
This work was supported in part by Grants-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan to SH (17052007 and 19040009).
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Kato, M., Takehana, Y., Sakaizumi, M. et al. A sex-determining region on the Y chromosome controls the sex-reversal ratio in interspecific hybrids between Oryzias curvinotus females and Oryzias latipes males. Heredity 104, 191–195 (2010). https://doi.org/10.1038/hdy.2009.114
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DOI: https://doi.org/10.1038/hdy.2009.114
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