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Volume 27 Issue 9, September 2020

Cover legend: Living on the edge. Left image: The transcription factor Oct4 (green) and cell surface marker SSEA4 (red) are classic markers of human induced pluripotent stem cells shown here at the edge of a colony, with DAPI showing both the nuclei of the stem cells as well as surrounding inactivated MEF feeder layer. Right image: Closer inspection reveals rapid cell division in these colonies as indicated by the frequent identification of condensed chromosomes undergoing mitosis and the temporary absence of the stem cell transcription factor. Images acquired by Dr Janine Scholefield, Bioengineering and Integrated Genomics, NextGen Health, CSIR, South Africa.

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