Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter to the Editor
  • Published:

EGFP-transduced EL-4 cells from tumors in C57BL/6 mice

Gene Therapy volume 8, pages 1814–1815 (2001)Cite this article

The immunogenicity of the enhanced green fluorescent protein (EGFP) has recently been demonstrated in mice.12 In particular, syngeneic tumor cells transduced with retroviral (RV) vectors expressing EGFP have been reported to be rejected following transplantation in immunocompetent C57BL/6 and BALB/c recipients.1 In the BALB/c mouse strain, the mechanism for rejection of CMS4 sarcoma and BM185 leukemia cells has been shown to be mediated by cytotoxic T-lymphocytes (CTLs) that recognize the foreign EGFP peptides.12 Furthermore, CTL responses have been observed in rhesus monkeys receiving a low nonmyeloablative dose of radiation and EGFP-transduced autologous hematopoietic cells.3

Attracted by the technical ease of using rejection in a solid tumor model for studying immunological tolerance, our laboratory has conducted a set of experiments using EL-4 T cell lymphoma cells growing in C57BL/6 mice, similar to those described by Stripecke et al.1 Briefly, EL-4 cells (ATCC, Rockville, MD, USA) were transduced with a RV vector denoted GBiP. This vector contains an EGFP (G) cDNA followed by the human immunoglobulin heavy chain binding protein (BiP) which is an internal ribosomal entry site (IRES) inserted in a pLN4 backbone modified with a myeloproliferative sarcoma virus (MPSV) enhancer in the 3’LTR.5 The plasmid pGBiP-6 was deposited with the ATCC (No. PTA 2498).

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1

References

  1. Stripecke R et al. Immune response to green fluorescent protein: implications for gene therapy Gene Therapy 1999 6: 1305–1312

    Article  CAS  PubMed  Google Scholar 

  2. Gambotto A et al. Immunogenicity of enhanced green fluorescent protein (EGFP) in BALB/c mice: identification of an H2-Kd-restricted CTL epitope Gene Therapy 2000 7: 2036–2040

    Article  CAS  PubMed  Google Scholar 

  3. Rosenzweig M et al. Induction of cytoxic T lymphocyte and antibody responses to enhanced green fluorescent protein following transplantation of transduced CD34+ hematopoietic cells Blood 2001 97: 1951–1959

    Article  CAS  PubMed  Google Scholar 

  4. Miller AD, Rosen GJ . Improved retroviral vectors for gene transfer and expression BioTechniques 1989 7: 980–990

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Banerjee PT et al. A polycistronic retrovirus vector for expression of swine MHC class II DRα/β heterodimers Xenotransplantation 1997 4: 161–173

    Article  Google Scholar 

Download references

Author information

Author notes

  1. G Andersson

    Present address: Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala BioMedical Center, Box 597, Uppsala, S-751 24, Sweden

Authors and Affiliations

  1. BioTransplant Incorporated, Building 75, Charlestown Navy Yard, Charlestown, 02129, MA, USA

    M Denaro, B Oldmixon, C Patience & J Down

Authors
  1. M Denaro
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B Oldmixon
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C Patience
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G Andersson
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J Down
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Denaro, M., Oldmixon, B., Patience, C. et al. EGFP-transduced EL-4 cells from tumors in C57BL/6 mice. Gene Ther 8, 1814–1815 (2001). https://doi.org/10.1038/sj.gt.3301601

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.gt.3301601

This article is cited by

Search

Advanced search

Quick links