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In vivo nuclear delivery of oligonucleotides via hybridizing bifunctional peptides

Gene Therapy volume 8, pages 84–87 (2001)Cite this article

Abstract

Linking proteins directly to nucleic acids has been a complex task. By hybridizing a bifunctional peptide nucleic acid (PNA) consisting of a nucleic acid binding moiety and a nuclear localization signal (NLS) we have previously demonstrated that it is possible to link protein functions directly to nucleic acids containing a PNA target site. By hybridizing fluorescently labeled oligonucleotides to PNA-NLS molecules and subsequently transfecting different organs in vivo we demonstrate an active nuclear translocation of the PNA-NLS/oligonucleotide complex in different mouse organs.

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Acknowledgements

This research has been supported by the Swedish Research Council.

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Authors and Affiliations

  1. Clinical Research Center, Karolinska Institutet, Huddinge University Hospital, Huddinge, Sweden

    LJ Brandén & CI Edvard Smith

  2. Department of Biosciences, Karolinska Institutet, NOVUM, Huddinge, Sweden

    LJ Brandén & CI Edvard Smith

  3. Department of Immunology, Microbiology, Pathology and Infectious Diseases, Huddinge University Hospital, Huddinge, Sweden

    LJ Brandén, B Christensson & CI Edvard Smith

Authors
  1. LJ Brandén
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  2. B Christensson
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  3. CI Edvard Smith
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Brandén, L., Christensson, B. & Smith, C. In vivo nuclear delivery of oligonucleotides via hybridizing bifunctional peptides. Gene Ther 8, 84–87 (2001). https://doi.org/10.1038/sj.gt.3301345

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  • DOI: https://doi.org/10.1038/sj.gt.3301345

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