Abstract
Previous studies have demonstrated that muscle can take up and express naked DNA or RNA. This study demonstrates that the pDNA can be delivered to and expressed within skeletal muscle when injected rapidly, in a large volume and when all blood vessels leading into and out of the hindlimb are occluded. The additional use of collagenase, papaverine and ischemia raised expression moderately but was not critical. These results demonstrate that a nonviral method can lead to high levels of expression in the muscles of adult animals larger than mice.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Budker, V., Zhang, G., Danko, I. et al. The efficient expression of intravascularly delivered DNA in rat muscle. Gene Ther 5, 272–276 (1998). https://doi.org/10.1038/sj.gt.3300572
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.gt.3300572
Keywords
This article is cited by
-
Current Status of Pharmaceutical and Genetic Therapeutic Approaches to Treat DMD
Molecular Therapy (2011)
-
Image-guided, Intravascular Hydrodynamic Gene Delivery to Skeletal Muscle in Pigs
Molecular Therapy (2010)
-
Gene doping: the hype and the reality
British Journal of Pharmacology (2008)
-
Computer-assisted Hydrodynamic Gene Delivery
Molecular Therapy (2008)