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Intravascular naked DNA vaccine encoding glycoprotein B induces protective humoral and cellular immunity against herpes simplex virus type 1 infection in mice

Gene Therapy volume 10, pages 2059–2066 (2003)Cite this article

Abstract

Naked plasmid DNA (pDNA) vaccine expressing herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) was tested for protective activity against acute HSV-1 infection in mice. The pDNA was intravenously injected into Balb/c mice via their tail vein under high pressure, and the vaccination was performed two times at an interval of 7 days. The gB gene vaccination significantly protected the mice from subsequent intraperitoneal challenge with a lethal dose of HSV-1, which killed all the animals given control plasmid or saline. The protective activity was correlated with the dose of the plasmid inoculated, the survival rate reaching 83% in mice vaccinated with 5 μg of pDNA. The vaccinated mice were also protected from latent HSV infection. The immunized mice showed significant elevation in neutralizing antibody against HSV-1 as well as serum levels of interleukin-12 (IL-12) and interferon-γ (IFN-γ). When mice were immunized with 5 μg of an Epstein–Barr virus (EBV)-based plasmid vector harboring the gB, the cytotoxic T lymphocytes (CTLs) activity and proliferative response for HSV-1 were also induced. The results strongly suggest that intravenous immunization of naked pDNA may induce humoral and cellular immune responses against the virus, leading to a significant prophylactic outcome against HSV-1 infection in mice.

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Acknowledgements

The present study was supported by a grant in aid from the Japanese Ministry of Education, Culture, Sports, Science and Technology.

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Authors and Affiliations

  1. Department of Microbiology, Kyoto Prefectural University of Medicine, Kyoto, Japan

    F-D Cui, H Asada, T Kishida, Y Itokawa, T Nakaya, M Kita, J Imanishi & O Mazda

  2. Department of Microbiology and Immunology, College of Medicine, Yanbian University, Yanji, China

    F-D Cui

  3. Department of Digestive Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan

    Y Itokawa, Y Ueda & H Yamagishi

  4. Department of Cardiovascular Surgery, Saitama Medical Center, Kawagoe, Japan

    S Gojo

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Cui, FD., Asada, H., Kishida, T. et al. Intravascular naked DNA vaccine encoding glycoprotein B induces protective humoral and cellular immunity against herpes simplex virus type 1 infection in mice. Gene Ther 10, 2059–2066 (2003). https://doi.org/10.1038/sj.gt.3302114

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