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A weak association of HLA-B*2702 with Behçet’s disease

Genes & Immunity volume 3, pages 368–372 (2002)Cite this article

Abstract

This study aimed to analyse the association of HLA-B alleles other than -B51 with Behçet’s disease (BD). We also investigated the frequency of HLA-B alleles sharing the same natural killer cell immunoglobulin-like receptor (KIR) binding sequence with HLA-B51. Broad-genotyping of HLA-B locus by PCR-SSOP in 174 Turkish BD patients and 191 healthy controls confirmed the strong association of B*51 with BD (60.9% in BD patients, 24.6% in healthy controls, OR = 4.78). No other HLA-B allele was identified showing an association with BD after adjusting for multiple testing or by using relative predispositional effects (RPE) analysis after the deletion of B*51. HLA-B alleles reacting with the sequence specific oligonucleotide probe 23, which corresponds to the KIR binding site of B*51, were found to be positive in 127 BD patients (73%) and 90 controls (47%) (OR = 3.03, 95% CI 2–4.7). The repeated RPE analysis after separating HLA-B alleles carrying B51-KIR binding sequence as distinct alleles within a broad-type allele group revealed B*2702 allele as the only allele showing an association with BD after the deletion of B*51. Selective increase of B*2702, the only B*27 allele carrying the same KIR binding sequence with B*51, warrants investigation of the possibility of interaction of HLA molecules with KIRs on NK or other T cells in the pathogenesis of BD.

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Acknowledgements

The authors thank Professor Alan J Silman for his critical reading and suggestions.

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Authors and Affiliations

  1. Division of Rheumatology, Department of Internal Medicine, Istanbul School of Medicine, University of Istanbul, Istanbul, Turkey

    A Gül, M Inanç, L Ocal, O Aral & M Koniçe

  2. Department of Physiology, Istanbul School of Medicine, University of Istanbul, Istanbul, Turkey

    F A Uyar & G Saruhan-Direskeneli

  3. ARC Epidemiology Unit, University of Manchester, Manchester, UK

    J H Barrett

Authors
  1. A Gül
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  2. F A Uyar
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  3. M Inanç
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  4. L Ocal
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  6. O Aral
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  7. M Koniçe
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  8. G Saruhan-Direskeneli
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Corresponding author

Correspondence to A Gül.

Additional information

This study was supported by University of Istanbul Research Funds, Project No. 1076/031297.

Appendix

Appendix

HLA-B alleles sharing aminoacid sequence of the B*51 between positions 77–82 of the α1-helix: B*0803, B*1513, B*1516, B*1517, B*1523, B*1524, B*1567, B*2702, B*3801, B*3805-B*3807, B*4013, B*4019, B*4406, B*4418, B*4425, B*4901-B*4903, B*5101-B*5127, B*5201-B*5203, B*5301, B*5302, B*5304, B*5306-B*5308, B*5701-B*5709, B*5801, B*5802, B*5804-B*5806, B*5901.

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Gül, A., Uyar, F., Inanç, M. et al. A weak association of HLA-B*2702 with Behçet’s disease. Genes Immun 3, 368–372 (2002). https://doi.org/10.1038/sj.gene.6363863

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