Abstract
Our study examined T cell receptor (TCR) Vβ mRNA expression in a murine model of experimental herpes simplex keratitis (HSK). We employed a polymerase chain reaction (PCR) technique to detect TCR Vβ mRNA expression in the inoculated eyes of both HSK-susceptible and HSK-resistant mice at different time points after corneal inoculation with herpes simplex virus type 1 (HSV-1), followed by Southern blotting and densitometry analysis. In eyes from HSK-susceptible C.AL-20 mice, a more diverse TCR Vβ transcript usage pattern was detected as compared with that seen in HSK-resistant C.B-17 mice. Vβ8 family members were expressed in both strains of mice at days 11, 14 and 21 post-inoculation. By densitometry, at day 11, the intensity of expression of Vβ8.2 and Vβ8.3 message was significantly greater in the eyes of C.AL-20 mice; Vβ8.1 was expressed only in C.B-17 mice. There were obvious differences in the TCR Vβ expression between HSK-susceptible and HSK-resistant mice. The differences in the intensity of the message expressed by Vβ8 family members between the two strains could be correlated to previous experiments that showed Vβ8.1,2+ T cells as the main infiltrating cells in the corneas of HSK-susceptible mice by day 11 and 14 after challenge with HSV-1.
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Pedroza-Seres, M., Goei, S., Merayo-Lloves, J. et al. T cell receptor vβ gene expression in experimental herpes stromal keratitis. Eye 9, 599–604 (1995). https://doi.org/10.1038/eye.1995.147
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DOI: https://doi.org/10.1038/eye.1995.147