Laboratory Study
Eye (2007) 21, 812–818; doi:10.1038/sj.eye.6702357; published online 2 June 2006
Short-term effect of intracameral triamcinolone acetonide on corneal endothelium using the rabbit model
None of the authors has a financial or proprietary interest in any of the material or method mentioned
Presented in part at the ASCRS Symposium, Washington DC, USA, April 2005
J Y Oh1, W R Wee2, J H Lee1 and M K Kim1
- 1Department of Ophthalmology, Seoul National University College of Medicine, Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Korea
- 2Seoul National University, Bundang Hospital, Seongnam, Korea
Correspondence: MK Kim, Department of Ophthalmology, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-744, Korea. Tel.: +82 2 2072 2665; Fax: +82 2 741 3187; E-mail: kmk9@medimail.co.kr
Received 17 November 2005; Accepted 16 March 2006; Published online 2 June 2006.
Abstract
Purpose
To investigate the effect of intracameral injection of triamcinolone acetonide on the corneal endothelium in rabbit eyes.
Methods
Triamcinolone acetonide (40 mg/ml, 0.2 cm3) after filtering and resuspension in balanced salt solution (BSS) was injected intracamerally for 3 min into 10 rabbit eyes and irrigated with 5 cm3 of BSS. Triamcinolone without resuspension and BSS were injected, respectively, into five rabbit eyes. Endothelial toxicity was evaluated and compared by measurements of endothelial cell counts and central corneal thickness. The endothelial viability was determined using vital staining with alizarin red and trypan blue at 2 h after injection. The scanning electron microscopy (SEM) was performed in one cornea from each group.
Results
Endothelial cell counts and central corneal thickness following intracameral injection of triamcinolone acetonide did not significantly change when compared to controls. The mean percentage of viable endothelial cells was 99.50, 99.52, and 99.49% in the resuspended triamcinolone group, triamcinolone without resuspension group, and BSS group, respectively (P=0.46, Kruskall–Wallis test). But SEM showed reduced microvilli of endothelial surface in an eye of the triamcinolone without resuspension group.
Conclusions
The intracameral injection of triamcinolone acetonide did not induce a significant visable change of endothelium in rabbit eyes. However, ultrastructural villi changes observed suggest a possibility of microstructural damages in endothelium with triamcinolone acetonide injection when used without filtering and resuspension.
Keywords:
cornea, effect, endothelium, toxicity, triamcinolone
