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Relationship between methylmalonic acid, homocysteine, vitamin B12 intake and status and socio-economic indices, in a subset of participants in the British National Diet and Nutrition Survey of people aged 65 y and over

European Journal of Clinical Nutrition volume 57, pages 349–357 (2003)Cite this article

Abstract

Objective: Assessment of functional vitamin B12 status in a subset of the respondents in the British National Diet and Nutrition Survey of people aged 65 y and over.

Setting: National Diet and Nutrition Survey: a British nationwide cross-sectional sample of people aged 65 y and over, living either in the community or in institutions such as nursing homes, during one calendar year spanning 1994–1995.

Methods: Methylmalonic acid (MMA) concentrations were measured in plasma samples from 313 subjects (ca 14% of those originally enrolled in the survey). The results were compared with those for serum vitamin B12, vitamin B12 intakes and other status and intake estimates and with socio-demographic indices.

Results: Of the NDNS participants overall, 20% had serum vitamin B12 concentrations<150 pmol/l. In the subset studied here, 24% of free-living and 46% of institution-living participants had MMA>0.5 µmol/l. Geometric mean MMA increased with age, from 0.25 µmol/l in people aged 65–74 y to 0.38 µmol/l in people aged 85+y. There was little evidence for any gender difference in MMA. It was inversely correlated with serum vitamin B12 and with red blood cell folate; it was positively correlated directly with total homocysteine, but not significantly with serum folate or with vitamin B12 intake. Among respondents with high MMA, a subgroup had normal serum vitamin B12 but higher-than-average plasma urea and creatinine. Socio-demographic co-variates of MMA included receipt of State income benefits, social class of head of household, and educational attainment. These indices were not correlated with serum vitamin B12.

Conclusions: The progressive increase in MMA with age is metabolic evidence for increasing risk of functional vitamin B12 deficiency with increasing age in older people. There is evidence that renal function is linked to high MMA in some older people. Age and renal function are thus both important when establishing upper reference limits for MMA. The socio-demographic observations suggest a link between poverty and poor functional vitamin B12 status in older British people.

Sponsorship: The Department of Health.

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Acknowledgements

The survey was commissioned jointly by the Department of Health and the Ministry of Agriculture, Fisheries and Food, whose responsibility has since been transferred to the Food Standards Agency. It was carried out by Social and Community Planning Research (now the National Centre for Social Research), in conjunction with the Micronutrient Status Laboratory of the MRC Dunn Nutrition Unit (now part of MRC Human Nutrition Research). Funding for the measurement of MMA was provided by the Department of Health.

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Author notes

  1. C J Bates: Guarantors: Dr CJ Bates.

  2. C J Bates, J Schneede, G Mishra, A Prentice and M A Mansoor: Contributors: CJB co-ordinated the analyses, contributed to data-analysis and interpretation and drafted the manuscript; JS and MAM performed the methylmalonic acid analyses and contributed to the interpretation; MAM performed the homocysteine analyses; GM provided statistical advice and AP directed the project in Cambridge, contributed to the study design and contributed to the interpretation.

Authors and Affiliations

  1. MRC Human Nutrition Research, Elsie Widdowson Laboratory, Cambridge, UK

    C J Bates, G Mishra & A Prentice

  2. Department of Pharmacology and Toxicology, University of Bergen, Haukeland Hospital, Bergen, Norway

    J Schneede

  3. Department of Clinical Chemistry, Central Hospital in Rogaland, Stavanger, Norway

    M A Mansoor

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Correspondence to C J Bates.

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Bates, C., Schneede, J., Mishra, G. et al. Relationship between methylmalonic acid, homocysteine, vitamin B12 intake and status and socio-economic indices, in a subset of participants in the British National Diet and Nutrition Survey of people aged 65 y and over. Eur J Clin Nutr 57, 349–357 (2003). https://doi.org/10.1038/sj.ejcn.1601540

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