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Significant up-regulation of a novel gene, CLCP1, in a highly metastatic lung cancer subline as well as in lung cancers in vivo

Oncogene volume 21, pages 2822–2828 (2002)Cite this article

Abstract

Most lung cancer patients are unfortunately uncurable and die because of widespread metastases, thus indicating the importance of identification of molecules with a crucial role in this process. Our previous expression profiling analysis of a highly metastatic lung cancer cell line, NCI-H460-LNM35, and its parental low metastatic line, NCI-H460-N15, revealed significant up-regulation of both known and unknown genes in LNM35. In this study, we describe the isolation and detailed characterizations of a novel gene, named CLCP1, which corresponds to one of such expression sequence tags with up-regulated expression in LNM35. The CLCP1 gene was found to encode a protein with 775 amino acids with structural similarities to, but distinct from neuropilins, cell surface receptors for VEGF165 and semaphorins. Notably, CLCP1 was shown to be up-regulated not only in LNM35 in association with its acquisition of metastatic phenotype during in vivo selection, but also in a significant fraction of lung cancers in vivo with high frequency in metastatic lesions, warranting future studies for a better understanding of the molecular mechanisms of lung cancer metastasis.

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Acknowledgements

The GenBank accession number for the CLCP1 cDNA is AB073146. This work was supported in part by a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

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Author notes

  1. Ken-ichi Kozaki

    Present address: Department of Pathology, Aichi Medical University School of Medicine, Nagakute, 480-1195, Aichi, Japan

Authors and Affiliations

  1. Division of Molecular Oncology, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa-ku, Nagoya, 464-8681, Japan

    Katsumi Koshikawa, Hirotaka Osada, Ken-ichi Kozaki, Hiroyuki Konishi, Akira Masuda, Yoshio Tatematsu & Takashi Takahashi

  2. Department of Thoracic Surgery, Aichi Cancer Center Hospital, 1-1 Kanokoden, Chikusa-ku, Nagoya, 464-8681, Japan

    Tetsuya Mitsudomi

  3. Department of Surgery II, Nagoya University School of Medicine, Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan

    Katsumi Koshikawa & Akimasa Nakao

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  1. Katsumi Koshikawa
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  2. Hirotaka Osada
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Correspondence to Takashi Takahashi.

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Koshikawa, K., Osada, H., Kozaki, Ki. et al. Significant up-regulation of a novel gene, CLCP1, in a highly metastatic lung cancer subline as well as in lung cancers in vivo. Oncogene 21, 2822–2828 (2002). https://doi.org/10.1038/sj.onc.1205405

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