Abstract
Objective:
To explore soy intake and urinary isoflavonoid excretion within several generations of American-Japanese women based on the hypothesis that earlier generations excrete higher levels of urinary isoflavonoids, in particular the metabolite equol, than later generations.
Subjects:
A convenience sample of 43 women from 19 families aged 18–78 years, all of whom reported at least 50% Japanese ancestry.
Interventions:
Each woman collected overnight urine samples at baseline and after consuming one serving of soymilk, both samples were analyzed for the isoflavonoids daidzein, genistein and equol using liquid chromatography–mass spectrometry.
Results:
Median isoflavone intakes during the last year were 7.2 mg/day for the first generation, 7.3 mg/day for the second generation and 6.3 mg/day for the third generation (P=0.36). At baseline, the median isoflavonoid excretion for the first generation was nonsignificantly higher than for later generations (190, 86 and 42 nmol/h; P=0.20) but after intervention, the median urinary isoflavonoid excretion was very similar for the three groups: 2465, 1895 and 2775 nmol/h (P=0.70). Following intervention, a nonsignificantly higher proportion of older than younger women (53 vs 32 and 33%; P=0.41) excreted the metabolite equol. The respective median equol excretion rates by generation following intervention were 39.5, 4.2 and 3.5 nmol/h (P=0.04).
Conclusions:
This small investigation among three generations of Japanese-Americans detected a higher equol production among older women after a soy challenge, but no difference in the excretion of total isoflavonoids after a standardized dose of soymilk was observed.
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References
Adams MR, Golden DL, Franke AA, Potter SM, Smith HS, Anthony MS (2004). Dietary soy beta-conglycinin (7S globulin) inhibits atherosclerosis in mice. J Nutr 134, 511–516.
Adlercreutz H (1995). Phytoestrogens: epidemiology and a possible role in cancer protection. Environ Health Perspect 103 (Suppl 7), 103–112.
Akaza H, Miyanaga N, Takashima N, Naito S, Hirao Y, Tsukamoto T et al. (2004). Comparisons of percent equol producers between prostate cancer patients and controls: case-controlled studies of isoflavones in Japanese, Korean and American residents. Jpn J Clin Oncol 34, 86–89.
Atkinson C, Berman S, Humbert O, Lampe JW (2004). In vitro incubation of human feces with daidzein and antibiotics suggests interindividual differences in the bacteria responsible for equol production. J Nutr 134, 596–599.
Atkinson C, Frankenfeld CL, Lampe JW (2005). Gut bacterial metabolism of the soy isoflavone daidzein: exploring the relevance to human health. Exp Biol Med (Maywood) 230, 155–170.
Atkinson C, Skor HE, Fitzgibbons ED, Scholes D, Chen C, Wahala K et al. (2002). Overnight urinary isoflavone excretion in a population of women living in the United States, and its relationship to isoflavone intake. Cancer Epidemiol Biomarkers Prev 11, 253–260.
Axelson M, Sjovall J, Gustafsson BE, Setchell KD (1984). Soya – a dietary source of the non-steroidal oestrogen equol in man and animals. J Endocrinol 102, 49–56.
Blair RM, Appt SE, Franke AA, Clarkson TB (2003). Treatment with antibiotics reduces plasma equol concentration in cynomolgus monkeys (Macaca fascicularis). J Nutr 133, 2262–2267.
Buell P (1973). Changing incidence of breast cancer in Japanese-American women. J Natl Cancer Inst 51, 1479–1483.
Chen Z, Zheng W, Custer LJ, Dai Q, Shu XO, Jin F et al. (1999). Usual dietary consumption of soy foods and its correlation with the excretion rate of isoflavonoids in overnight urine samples among Chinese women in Shanghai. Nutr Cancer 33, 82–87.
Cline JM, Franke AA, Register TC, Golden DL, Adams MR (2004). Effects of dietary isoflavone aglycones on the reproductive tract of male and female mice. Toxicol Pathol 32, 91–99.
Coward L, Barnes NC, Setchell KDR, Barnes S (1993). Genistein, daidzein, and their β-glycoside conjugates: antitumor isoflavones in soybean foods from American and Asian diets. J Agric Food Chem 41, 1961–1967.
Deapen D, Liu L, Perkins C, Bernstein L, Ross RK (2002). Rapidly rising breast cancer incidence rates among Asian-American women. Int J Cancer 99, 747–750.
Franke AA, Custer L, Hundahl S (2004). Determinants for urinary and plasma isoflavones in humans after soy intake. Nutr Cancer 50, 141–154.
Franke AA, Custer L, Wilkens L, LeMarchand L, Goodman MT, Kolonel LN (2002). LC/PDA/MS analysis of dietary phytoestrogens from human urine and blood. J Chromatogr B 777, 43–57.
Franke AA, Custer LJ, Wang W, Shi SJ (1998). HPLC analysis of isoflavonoids and other phenolic agents from foods and from human fluids. Proc Soc Exp Biol Med 217, 263–273.
Franke AA, Hankin JH, Yu MC, Maskarinec G, Low SH, Custer LJ (1999). Isoflavone levels in soy foods consumed by multiethnic populations in Singapore and Hawaii. J Agric Food Chem 47, 977–986.
Frankenfeld CL, Atkinson C, Thomas WK, Goode EL, Gonzalez A, Jokela T et al. (2004). Familial correlations, segregation analysis, and nongenetic correlates of soy isoflavone-metabolizing phenotypes. Exp Biol Med (Maywood) 229, 902–913.
Lampe JW, Gustafson DR, Hutchins AM, Martini MC, Li S, Wahala K et al. (1999). Urinary isoflavonoid and lignan excretion on a Western diet: relation to soy, vegetable, and fruit intake. Cancer Epidemiol Biomarkers Prev 8, 699–707.
Lampe JW, Karr SC, Hutchins AM, Slavin JL (1998). Urinary equol excretion with a soy challenge: influence of habitual diet. Proc Soc Exp Biol Med 217, 335–339.
Maskarinec G, Singh S, Meng L, Franke AA (1998). Dietary soy intake and urinary isoflavone excretion among women from a multiethnic population. Cancer Epidemiol Biomarkers Prev 7, 613–619.
Maskarinec G, Takata Y, Franke AA, Williams AE, Murphy SP (2004). A 2-year soy intervention in premenopausal women does not change mammographic densities. J Nutr 134, 3089–3094.
Murphy SP (2002). Unique nutrition support for research at the Cancer Research Center of Hawaii. Hawaii Med J 61, 15–17.
Nordyke EC (1989). The Peopling of Hawaii. University of Hawaii Press: Honolulu.
Ozasa K, Nakao M, Watanabe Y, Hayashi K, Miki T, Mikami K et al. (2004). Serum phytoestrogens and prostate cancer risk in a nested case–control study among Japanese men. Cancer Sci 95, 65–71.
Pike MC, Kolonel LN, Henderson BE, Wilkens LR, Hankin JH, Feigelson HS et al. (2002). Breast cancer in a multiethnic cohort in Hawaii and Los Angeles: risk factor-adjusted incidence in Japanese equals and in Hawaiians exceeds that in whites. Cancer Epidemiol Biomarkers Prev 11, 795–800.
Rowland IR, Wiseman H, Sanders TA, Adlercreutz H, Bowey EA (2000). Interindividual variation in metabolism of soy isoflavones and lignans: influence of habitual diet on equol production by the gut microflora. Nutr Cancer 36, 27–32.
SAS Institute Inc (2004). SAS OnlineDoc 9.1.2. SAS Institute Inc: Cary, NC.
Saunier K, Dore J (2002). Gastrointestinal tract and the elderly: functional foods, gut microflora and healthy ageing. Dig Liver Dis 34 (Suppl 2), S19–S24.
Seow A, Shi CH, Franke AA, Hankin H, Lee HP, Yu MC (1998). Isoflavonoid levels in spot urine predict frequency of dietary soy intake in a population-based sample of middle-aged Chinese in Singapore. Cancer Epidemiol Biomarkers Prev 7, 135–140.
Setchell KD, Brown NM, Desai PB, Zimmer-Nechimias L, Wolfe B, Jakate AS et al. (2003). Bioavailability, disposition, and dose–response effects of soy isoflavones when consumed by healthy women at physiologically typical dietary intakes. J Nutr 133, 1027–1035.
Setchell KD, Brown NM, Lydeking-Olsen E (2002). The clinical importance of the metabolite equol – a clue to the effectiveness of soy and its isoflavones. J Nutr 132, 3577–3584.
Shimizu H, Ross RK, Bernstein L, Yatani R, Henderson BE, Mack TM (1991). Cancers of the prostate and breast among Japanese and white immigrants in Los Angeles County. Br J Cancer 63, 963–966.
Shu XO, Jin F, Dai Q, Wen W, Potter JD, Kushi LH et al. (2001). Soyfood intake during adolescence and subsequent risk of breast cancer among Chinese women. Cancer Epidemiol Biomarkers Prev 10, 483–488.
Song KB, Atkinson C, Frankenfeld CL, Jokela T, Wahala K, Thomas WK et al. (2006). Prevalence of daidzein-metabolizing phenotypes differs between Caucasian and Korean American women and girls. J Nutr 136, 1347–1351.
Tsukamoto C, Shimada S, Igita K, Kudou S, Kokubun M, Okubo K et al. (1995). Factors affecting isoflavone content in soybean seeds: changes in isoflavones, saponins, and composition of fatty acids at different temperatures during seed development. J Agric Food Chem 43, 1184–1192.
van Tongeren SP, Slaets JP, Harmsen HJ, Welling GW (2005). Fecal microbiota composition and frailty. Appl Environ Microbiol 71, 6438–6442.
Wang H, Murphy PA (1994). Isoflavone content in commercial soybean foods. J Agric Food Chem 42, 1666–1673.
Williams AE, Maskarinec G, Hebshi S, Oshiro C, Murphy S, Franke AA (2003). Validation of a soy questionnaire with repeated dietary recalls and urinary isoflavone assessments over one year. Nutr Cancer 47, 118–125.
Wu AH, Wan P, Hankin J, Tseng CC, Yu MC, Pike MC (2002). Adolescent and adult soy intake and risk of breast cancer in Asian- Americans. Carcinogenesis 23, 1491–1496.
Yan L, Spitznagel E (2004). A meta-analysis of soy foods and risk of breast cancer in women. Int J Cancer Prev 1, 281–293.
Ziegler RG, Hoover RN, Pike MC, Hildesheim A, Nomura MY, West DW et al. (1993). Migration patterns and breast cancer risk in Asian-American women. J Natl Cancer Inst 85, 1819–1827.
Zubik L, Meydani M (2003). Bioavailability of soybean isoflavones from aglycone and glucoside forms in American women. Am J Clin Nutr 77, 1459–1465.
Acknowledgements
We are grateful to the Meiji Foundation for supporting this project, the study participants and Laurie Custer for her analysis of the urine samples.
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Maskarinec, G., Yamakawa, R., Hebshi, S. et al. Urinary isoflavonoid excretion and soy consumption in three generations of Japanese women in Hawaii. Eur J Clin Nutr 61, 255–261 (2007). https://doi.org/10.1038/sj.ejcn.1602511
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DOI: https://doi.org/10.1038/sj.ejcn.1602511
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