Abstract
Molecular barcode arrays allow the analysis of thousands of biological samples in parallel through the use of unique 20-base-pair (bp) DNA tags. Here we present a new barcode array, which is unique among microarrays in that it includes at least five replicates of every tag feature. The use of smaller dispersed replicate features dramatically improves performance versus a single larger feature and allows the correction of previously undetectable hybridization defects.
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Acknowledgements
This work was supported by a grant from the US National Human Genome Research Institute. We thank B. St. Onge, M. Hillenmeyer and S. Brachat for suggestions.
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Supplementary information
Supplementary Fig. 1
Use of unassigned tag probes to estimate background hybridization. (PDF 136 kb)
Supplementary Figure 2
Effect of repairs on tag performance. (PDF 237 kb)
Supplementary Table 1
Detailed information on the repaired tags. (PDF 160 kb)
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Pierce, S., Fung, E., Jaramillo, D. et al. A unique and universal molecular barcode array. Nat Methods 3, 601–603 (2006). https://doi.org/10.1038/nmeth905
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DOI: https://doi.org/10.1038/nmeth905
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