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The charged sialic acid exhibits its power again: a new high-throughput screening technology

In this issue, the Withers laboratory takes advantage of the negatively charged sialic acid to develop a high-throughput screening technology that can be used for evolving glycosyltransferases with new enzymatic activities.

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Figure 1: High-thoughput screening methodology for evolving sialyltransferases.

Simon Fenwick

References

  1. Varki, A. et al., eds. in Essentials of Glycobiology (CSHL Press, Cold Spring Harbor, 1999).

    Google Scholar 

  2. Kim, Y.W., Lee, S.S., Warren, R.A. & Withers, S.G. J. Biol. Chem. 279, 42787–42793 (2004).

    Article  CAS  Google Scholar 

  3. Hauser, S., Song, H., Li, H. & Wang, L.X. Biochem. Biophys. Res. Commun. 328, 580–585 (2005).

    Article  CAS  Google Scholar 

  4. Lin, H., Tao, H. & Cornish, V.W. J. Am. Chem. Soc. 126, 15051–15059 (2004).

    Article  CAS  Google Scholar 

  5. Aharoni, A. et al. Nat. Methods 3, 609–614 (2006).

    Article  CAS  Google Scholar 

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Wang, P. The charged sialic acid exhibits its power again: a new high-throughput screening technology. Nat Methods 3, 589–590 (2006). https://doi.org/10.1038/nmeth0806-589

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